中文摘要：

目的观察L02细胞经饮用水有机提取物染毒后所诱发的白介素6（interleukin 6,IL-6）和丙氨酸氨基转移酶（alanine transaminase,ALT）及细胞凋亡的改变。方法采用固相萃取法提取饮用水中有机污染物。将取处于对数生长期的正常人肝（L02）细胞分别暴露于分别暴露于含终浓度0.312 5、0.625 0、1.250 0、2.500 0、5.000 0 L/ml有机提取物的培养液中暴露24、48、72 h,同时设溶剂对照（含0.1%DMSO培养液）和空白对照（培养液）。检测细胞凋亡率及细胞培养液上清中IL-6的含量和ALT的活力。结果与溶剂对照组比较,各剂量饮用水有机提取物染毒不同时间L02细胞的凋亡率均较高,除0.312 5、0.625 0 L/ml染毒24 h和0.312 5 L/ml染毒48 h组外,差异均有统计学意义（P〈0.05）;各剂量饮用水有机提取物染毒不同时间L02细胞培养液上清中IL-6的含量均较高,差异均有统计学意义（P〈0.05）;各剂量饮用水有机提取物染毒不同时间L02细胞培养液上清中ALT的活力均较高,除0.312 5、0.625 0 L/ml染毒24 h组外,差异均有统计学意义（P〈0.05）。且随着饮用水有机提取物染毒剂量的升高和染毒时间的延长,L02细胞的凋亡率及细胞培养液上清中IL-6含量和ALT活力均呈上升趋势。结论在本实验条件下,饮用水有机污染物可剂量依赖性及时间依赖性地诱导L02细胞炎性因子IL-6的分泌,引发炎症反应,诱导细胞凋亡,从而导致肝细胞损伤。

英文摘要：

Objective To observe the effect of organic extracts from drinking water on interleukin 6（IL-6）,alanine transaminase（ALT） and apoptosis in hepatocytes（L02 cells）. Methods Organic pollutants in water samples were extracted by solid phase extraction method. L02 cell were divided into seven groups： blank control group（culture medium）,solvent control group（0.1%DMSO） and organic extracts groups（the dose of extracts groups were 0.312 5,0.625 0,1.250 0,2.500 0 and 5.000 0 L/ml respectively）. Each group was treated for 24 h, 48 h and 72 h respectively. The rate of cell apoptosis was detected with flow cytometry. ELISA was selected to determine the concentrations of IL-6 in the supernatant. The activity of ALT was detected with automatic biochemical analyzer. Results Compared with the solvent control group,the apoptosis rates in organic extracts groups at different time points were obviously higher except for 0.312 5, 0.625 0 L/ml at 24 h and 0.312 5 L/ml at 48 h（P〈0.05）; The IL-6 levels in organic extracts exposure group at different time points were obviously higher（P〈0.05）. The activities of ALT in organic extracts exposure group at different time points were obviously higher except for 0.312 5,0.625 0 L/ml at24 h（P〈0.05）. With the increasing of organic extracts level and exposure time,the apoptosis rates,the IL-6 levels and the ALT activities in organic extracts exposure groups were obviously higher than control group（P〈 0.05）. Conclusion The organic extracts from drinking water can dose-dependently or time-dependently induce the secretion of IL-6 in L02 cells and induce hepatocytes apoptosis.