中文摘要：

目的：建立同时测定丹参中2种水溶性和3种脂溶性成分的HPLC方法。方法：采用C18柱（4.6 mm×250 mm,5μm）,以乙腈（A）-0.05%磷酸水溶液（B）为流动相进行梯度洗脱：0～27 min,20%～25%A;27～55 min,25%～80%A;55～65min,80%～80%A;检测波长为270 nm和286 nm,柱温30℃。结果：迷迭香酸、丹酚酸B、隐丹参酮、丹参酮Ⅰ、丹参酮ⅡA分别在1.92×10-2～0.96μg,7.29×10-2～3.64μg,3.05×10-3～0.15μg,1.71×10-3～8.55×10-2μg,5.80×10-3～0.29μg呈良好的线性关系。加样回收率分别为99.88%,104.75%,101.08%,101.41%,102.41%。结论：该含量测定方法准确、稳定、简便,分离效果好,能同时测定丹参中迷迭香酸、丹酚酸B、隐丹参酮、丹参酮Ⅰ、丹参酮ⅡA 5种有效成分的含量。

英文摘要：

Objective： To develop a new method for simultaneous determination of two hydrophillc components and three lipophillc components of Radix et Rhizoma Salviae Miltiorrhizae.Method：The HPLC-double wavelength detection was employed using a column of Dikma Diamonsil C18（4.6 mm × 250 mm,5 μm） with a mobile phase of acetonitrile（A）-0.05% phosphoric acid（B）.The gradient elution program was as follows：0-27 min,20%-25% A;27-55 min,25%-80% A;55-65 min,80%-80% A.The detection wavelength was set at 270 nm and 286 nm.The column temperature was at 30 ℃.Result：Rosmarinic acid was linear in the range of 1.92 × 10-2-0.96 μg;salviamolic acid B was linear in the range of 7.29 × 10-2-3.64 μg;clyptotanshinon was linear in the range of 3.05 × 10-3-0.15 μg;tanshinone was linear in the range of 1.71 × 10-3-8.55 × 10-2 μg and tanshinone ⅡA was linear in the range of 5.80 × 10-3-0.29 μg.The average recovery was 99.88%,104.75%,101.08%,101.41%,102.41% respectively.Conclusion：The present method was simple and had satisfactory efficacy;it can simultaneously determine multiple hydrophilic and lipophilic bioactive components in Salvia miltiorrhiza from different areas.