中文摘要：

目的：建立兔血浆中2种蟾蜍甾烯类化合物蟾毒灵及酯蟾毒配基的分析方法,探讨其在兔体内的药代动力学过程。方法：兔按0.35 mg.kg-1由耳缘静脉注射蟾酥提取物后分别于2,5,10,15,20,30,45,60,90 min心脏取血,采用乙腈沉蛋白与液液萃取相结合方法进行血浆样品预处理,以HPLC测定兔血浆中蟾毒灵及酯蟾毒配基的浓度,以Kinetica软件拟合药动学参数。结果：建立的HPLC方法,蟾毒灵、酯蟾毒配基均得到很好的分离,达到体内分析要求。经非房室模型拟合,得到了蟾毒灵、酯蟾毒配基在兔体内主要药动学参数。结论：建立的蟾毒灵、酯蟾毒配基血药浓度测定方法及所获得的药动学参数,可为中药蟾酥提取物的药代动力学研究提供参考。

英文摘要：

Objective： To establish a HPLC method for the assay of bufalin （ BL） ,resibufogenin （ RBG）and study the pharmacokinetics of BL,RBG in rabbits plasma. Method： The sample blood from rabbits were injected venenum bufonis extract by 0. 35 mg.kg -1at 2,5,10,15,20,30,45,60,90 min. Protein precipitation with acetonitrile and liquid-liquid extraction was applied to purify plasma samples and used HPLC to determine the concentration of BL,RBG in it. The pharmacokinetics parameters were accessed by Kinetica software. Result： In this HPLC method,the separation,precision and accuracy of BL,RBG were well. The calibration curves were linear. The analytical recoveries were all more than 90% ,intra and inter-day precision RSD were all less than15. 0% （ n = 5） . The main pharmacokinetics parameters were fitted by non-compartment models. Conclusion： The HPLC method can be used to determine the concentration and to investigate the pharmacokinetics of BL and RBG.