中文摘要：

目的：建立同时测定延胡索中巴马汀、脱氢紫堇碱和延胡索乙素的HPLC方法。方法：采用C18柱（4.6 mm×250nm,5μm）,以乙腈（A）-0.1%磷酸（加三乙胺调节pH 6.0,B）进行梯度洗脱（0～20 min,25%A;20～30 min,25%～55%A;30～45 min,55%A;45～50 min,55%～25%A;50～55 min,25%A）;检测波长为270,280 nm,柱温30℃。结果：巴马汀在2.12×10-2～1.59μg呈良好的线性关系（r=0.999 9）;脱氢紫堇碱在4.10×10-2～3.07μg呈良好的线性关系（r=0.999 9）;延胡索乙素在2.10×10-2～1.57μg呈良好的线性关系（r=0.999 9）;加样回收率分别为100.10%,100.03%,97.47%。结论：该方法准确、稳定、分离效果好,可用于测定延胡索药材中巴马汀、脱氢紫堇碱和延胡索乙素的含量。

英文摘要：

Objective： To develop a new method dehydrocorydaline nitrate and tetrahydropalmatine in Rhizoma adopted performing on a C18column （4.6 mm ×250 for determination of palmatine hydrochloride, Corydalis by HPLC. Method： The HPLC was nm, 5 μm） with a mobile phase of acetonitrile （A） 43.1%phosphoric acid （B）. The gradient elution program was as follows： 0-20 min, 25% A; 20-30 min, 25%-55% A; 30-45 min, 55%A; 45-50 min, 55%-25%A; 50-55 min, 25%A; The detection wavelength was set at270 nm and 280 nm. The column temperature was at 30 ℃. Result： Palmatine hydrochloride was linear in the range of 2. 12 × 10^-2 -1.59 μg （r =0. 999 9） ; dehydrocorydaline nitrate was linear in the range of 4. 10 ×10^-2 -3.07 μg （r =0. 999 9） ; tetrahydropalmatine was linear in the range of 2. 10× 10^-2 -1. 57 μg （r =0. 999 9）. The average recovery was 100. 10%, 100.03% and 97.47%. Conclusion： The method was accurate and had satisfactory efficacy; it can determine palmatine hydrochloride, dehydrocorydaline nitrate and tetrahydropalmatine in Rhizoma Corydalis.