中文摘要：

通过控制驯化条件对接种物进行选择性培养,分析接种物驯化过程中pH值、SCOD质量浓度、辅酶F420质量摩尔浓度和产气量的变化.获得接种物的驯化方案为：在35℃培养温度下,每天进料量为2g泥炭,驯化30d;其在第20天辅酶F420质量摩尔浓度达到最高值0.037 3mmol/g,远远大于对照组P0第19天的辅酶F~（420）质量摩尔浓度最高值0.011 0mmol/g,揭示驯化接种物有助于富集厌氧微生物,尤其是产甲烷细菌.实验结果可为泥炭转化为生物甲烷、扩大煤制天然气煤种资源开辟新途径.

英文摘要：

The inoculum was selective culture by controlling the domesticated conditions. The changes of pH, SCOD and co-F420 of sludge in the process of domestication, as well as the daily gas production and total gas production were analyzes. The results show the optimal domestication plan coenzyme for inoculum is 35 ℃ temperature, feed rate of 2 g/d, and domestication time 30 d. The maximum value of co-F420 is 0. 037 3 mmol/g on the 20th day, far greater than that of the control group P0 0. 011 0 mmol/g on the 19th day, which reveals that the domestica- ted inoculum can help anaerobic microorganisms enrichment, especially the methanogenic bacteria. This experiment can open up new ways to peat transformed into biomethane, expand additional coal resources for coal gasification.