中文摘要：

目的观察细粒棘球蚴egG1Y162疫苗免疫小鼠后机体的免疫应答状况。方法实验组、GST对照组、佐剂组和正常组小鼠分别注射egG1Y162疫苗、谷胱甘肽巯基转移酶（GSTs）、佐剂（FCA）和生理盐水（NS），在免疫第0、2、4、6、8和10周，收集各组血清用酶联免疫吸附法（ELISA法）检测抗体滴度，并检测各组血清抗体IgG的动态变化。第10周用四甲基偶氮唑蓝实验（MTT法）检测免疫小鼠脾淋巴细胞体外刺激后的增殖反应，用AnnexinV—FITC和碘化丙啶（PI）双染色法检测脾细胞凋亡率。结果egG1Y162疫苗免疫组小鼠在第2周免疫后检测到抗体，在免疫第10周，抗体滴度可达到1：25600。血清抗体IgG在免疫第2～10周不断升高，并在免疫第10周达到最高水平。MTT法显示egG1Y162疫苗免疫组小鼠脾淋巴细胞在体外能被egG1Y162疫苗特异性刺激增生。疫苗剌激后疫苗组增殖水平显著高于对照组、佐剂组和正常组（P〈0．05）。AnnexinV—FITC和PI双染色法结果显示实验组小鼠脾细胞原液和ConA刺激细胞凋亡发生率均显著低于对照组（P〈0．01），每组ConA刺激脾细胞凋亡发生率显著高于相应的原液培养（P〈0．01）。结论细粒棘球绦虫egG1Y162疫苗可诱导小鼠产生高效价抗体并可刺激脾淋巴细胞增殖活化、抑制脾细胞凋亡，特异性抗体反应和脾淋巴细胞增殖活化促进机体产生体液免疫反应和细胞免疫反应，共同协调诱导机体产生免疫应答反应。

英文摘要：

The purpose of this study was to investigate the immunological effects induced by the egG1Y162 hydride vac- cine antigen in mice. The experimental group, GST group, FCA group and NS group of mice were injected with egG1Y162 vaccine, GST, FCA and NS. Sera were collected to determine the levels of IgG using ELISA. The proliferation of splenic T lymphocytes was determined by MTT method. Then the cells were collected and stained by PI and AnnexinV-FITC, and then analyzed by FCM to obtain the apoptotic rate of splenic T lymphocytes. It was found that the specific IgG response was induced during the second week after immune and continued to increase until 10th week. The highest titer of specific antibodies was 1 ： 25 600 in sera of mice immunized with the egG1Y162 antigen. Level of IgG in the sera of mice increased obviously on 2-10 weeks, reaching the highest level on 10^th week. As demonstrated by the MTT assay, the egG1Y162 vaccine could specifically stimulate the proliferation of splenic T lymphocytes in mice. In the statistical analysis, the levels of proliferation stimulation af- ter vaccination in the vaccine group were significantly higher than those in the control, adjuvant, and normal groups. Apoptotic rate of splenic T lymphocytes in immunization groups with or without ConA stimulation was obviously lower than that of PBS group （P~0. 01）. Apoptotic rate of splenic T lymphocytes in each group with ConA stimulation was greatly higher than that in the group without ConA stimulation （P〈0.01）. The Echinococcus granulosus egG1Y162 vaccine could induce high titers ofantibodies and stimulate spleen lymphocytes＇ specific prolif eration activation. Results indicated that the specific antibod- ies response and splenic proliferation activation collaboratively promote the production of humoral immune and cellular im- mune responses, which jointly induce the production of im- mune responses.