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中文摘要：
目的: Gelsemine，从中国植物 Gelsemium elegans 的碱(Gardn 与捣烂) Benth，在在老鼠减轻长期的疼痛是有效的。在我们调查了碱是否改进了睡觉骚乱的现在的学习，长期的疼痛的最普通的 comorbid 症状，在神经病的 pain.Methods 的一个老鼠模型: 老鼠受到部分臀部的神经结扎(PSNL ) 。在老鼠 intraperitoneally 与 gelsemine 或 pregabalin (积极控制) 被注射以后，机械 allodynia 和热痛觉过敏被估计，并且记录的脑电图(EEG )/electromyogram (EMG ) 被执行。鼠标的马达性能用花名册杆测试被估计。在大脑的 c-Fos 表示与 immunohistochemical staining.Results 被分析: 在 PSNL 老鼠， gelsemine (2 和 4 mg/kg ) 为 4 h 增加了机械阀值并且为 3 h 延长了热潜伏。而且， gelsemine (4 mg/kg，在上午 6:30 管理了) 增加的非快速的眼睛运动(非雷姆， NREM ) 睡觉，减少的醒着，但是没在 PSNL 老鼠在开始的 3 h 期间影响雷姆睡觉。睡觉建筑学分析证明 gelsemine 减少了醒着的吝啬的持续时间并且在 dosing 以后在开始的 3 h 期间增加了 NREM 睡觉的事件的全部的数字。Gelsemine (4 mg/kg ) 没在 PSNL 老鼠损害马达协作。Immunohistochemical 学习证明 PSNL 在前面的 cingulate 外皮的神经原增加了 c-Fos 表示，并且 gelsemine (4 mg/kg ) 减少了在 58% 的 c-Fos 表示。Gelsemine (4 mg/kg，在上午 6:30 或下午 8:30 管理了) 没在正常老鼠生产催眠效果。Pregabalin 生产了类似的 antinociceptive 和催眠效果，但是在 PSNL mice.Conclusion 损害了马达协作: Gelsemine 在 PSNL 老鼠是为神经病的疼痛和睡觉骚乱的治疗的一个有效代理人；前面的 cingulate 外皮可能在 gelsemine 的催眠效果起一个作用。
英文摘要：
Aim： Gelsemine, an alkaloid from the Chinese herb Gelsemium elegans （Gardn ＆ Champ） Benth, is effective in mitigating chronic pain in rats. In the present study we investigated whether the alkaloid improved sleep disturbance, the most common comorbid symptoms of chronic pain, in a mouse model of neuropathic pain. Methods： Mice were subjected to partial sciatic nerve ligation （PSNL）. After the mice were injected with gelsemine or pregabalin （the positive control） intraperitoneally, mechanical allodynia and thermal hyperalgesia were assessed, and electroencephalogram （EEG）/ electromyogram （EMG） recording was performed. Motor performance of the mice was assessed using rota-rod test. c-Fos expression in the brain was analyzed with immunohistochemical staining. Results： In PSNL mice, gelsemine （2 and 4 mg/kg） increased the mechanical threshold for 4 h and prolonged the thermal latencies for 3 h. Furthermore, gelsemine （4 mg/kg, administered at 6：30 AM） increased non-rapid eye movement （non-REM, NREM） sleep, decreased wakefulness, but did not affect REM sleep during the first 3 h in PSNL mice. Sleep architecture analysis showed that gelsemine decreased the mean duration of wakefulness and increased the total number of episodes of NREM sleep during the first 3 h after the dosing. Gelsemine （4 mg/kg） did not impair motor coordination in PSNL mice. Immunohistochemical study showed that PSNL increased c-Fos expression in the neurons of the anterior cingulate cortex, and gelsemine （4 mg/kg） decreased c-Fos expression by 58%. Gelsemine （4 mg/kg, administered at either 6：30 AM or 8：30 PM） did not produce hypnotic effect in normal mice. Pregabalin produced similar antinociceptive and hypnotic effects, but impaired motor coordination in PSNL mice. Conclusion： Gelsemine is an effective agent for treatment of both neuropathic pain and sleep disturbance in PSNL mice; anterior cingulate cortex might play a role in the hypnotic effects of gelsemine.
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