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贮藏蛋白组分对小麦面团流变学特性和食品加工品质的影响
  • ISSN号:0578-1752
  • 期刊名称:中国农业科学
  • 时间:0
  • 页码:2937-2946
  • 语言:中文
  • 分类:S512.1[农业科学—作物学] S565.201[农业科学—作物学]
  • 作者机构:[1]中国农业科学院作物科学研究所/国家农作物基因资源与基因改良重大科学工程/国家小麦改良中心,北京100081, [2]新疆农业大学农学院,乌鲁木齐830052, [3]山东省农业科学院作物研究所,济南250100, [4]江苏省农业科学院生物技术研究所,南京210014, [5]国际玉米小麦改良中心中国办事处,北京100081
  • 相关基金:国家自然科学基金项目(30600393);国家重点基础研究发展计划(973计划)项目(2002CB111300):“863”重大专项(2006AA100102);国家“十一五”科技支撑计划项目(2006BAD01A02)和“948”重大国际合作项目(2006-G2)
  • 相关项目:高分子量麦谷蛋白亚基表达量的PQL分析及其与面筋强度的关系
作者: 张勇|
中文摘要:

【目的】贮藏蛋白组成与组分含量显著影响食品加工品质,研究贮藏蛋白组分含量对加工品质的影响,指导小麦品质改良。【方法】选用近几年北方冬麦区育成的优质品种和高代品系及山东省主栽品种为材料,采用反相高效液相色谱(RP-HPLC)和凝胶色谱(SE-HPLC)方法对贮藏蛋白组分进行量化,并分析它们与面团流变学特性、面包、面条和馒头加工品质的关系。【结果】谷蛋白总量(Glu)、高分子量麦谷蛋白亚基(HMW-GS)含量和低分子量麦谷蛋白亚基(LMW-GS)含量与反映面筋强度的和面时间、稳定时间、拉伸面积和最大抗延阻力呈1%显著正相关(r = 0.61~0.83),与面包品质均呈显著正相关(r=0.34~0.85),而醇溶蛋白与谷蛋白含量比(Gli/Glu)与这些参数均呈显著负相关(r = -0.37~-0.85,P〈0.05)。SDS不溶性谷蛋白聚合体百分含量(%UPP)与面团稳定时间和最大抗延阻力呈对数关系,决定系数分别达0.84和0.86;与面包总分呈显著正相关(r=0.76,P<0.001),决定系数为0.58。Glu、HMW-GS、LMW-GS和%UPP与面条的色泽、硬度、总分和馒头的光滑度呈5%显著负相关(r=-0.36~-0.63),而Gli/Glu与这些参数呈5%显著正相关(r=0.37~0.57)。醇溶蛋白总量及各组分含量与馒头的压缩张驰性呈1%显著正相关,与馒头的总评分呈显著负相关。【结论】贮藏蛋白组分含量对面团流变学特性和面包品质影响较大,当Glu和%UPP分别达到32.5 AU和48.9%时,面筋强度(稳定时间大于10.0 min)和面包品质较优,而对面条和馒头品质影响相对较小,且多为负向效应。

英文摘要:

[Objective] The quantity of gluten protein fractions significantly influences wheat end-products quality, therefore, understanding of the effects of gluten protein fractions on products quality will be very useful for quality improvement. [ Method ] Totally, 43 wheat genotypes including newly released high quality cultivars and advanced lines in North China winter wheat region and leading cultivars in Shandong province, were grown in Jinan in 2005-2006. Their protein fractions were quantified by reversed-phase high-performance liquid chromatography (RP-HPLC) and size-exclusion high-performance liquid chromatography (SE-HPLC) methods to determine the relationship between protein fractions and dough properties and end-products. [ Result] The quantity of total glutenin, HMW-GS and LMW-GS were significantly and positively correlated with mixograph peak value, farinograph stability, extensograph extension area and maximum resistance, with r=0.61-0.83 (P〈0.001), and they were significantly and positively associated with all bread quality parameters, with r=0.34-0.85 (P〈0.05 or P〈0.001). But the ratio of Gli/Glu was significantly and negatively correlated with these parameters, with r=-0.37-0.85 (P〈0.05 or P〈0.001). The percent of SDS-unextractable glutenin polymeric protein (%UPP) showed natural logarithm correlation with dough stability and maximum resistance, R^2=0.84 and 0.86, respectively, %UPP was significantly and positively associated with bread total score, with r=-0.76 (P〈 0.001). The quantity of total glutenin, HMW-GS, LMW-GS and %UPP were significantly and negatively correlated with noodle color, firmness, total score and steamed bread smoothness, with r=-0.36-0.63 (P〈0.05 or P〈0.001), but the ratio of Gli/Glu was significantly and positively associated with these parameters, with r=-0.37-0.57 (P〈0.05). The quantity of total gliadin was significantly and positively related with steamed bread stress relaxation, with r=0.54(P〈0.001), but was significa

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期刊信息
  • 《中国农业科学》
  • 中国科技核心期刊
  • 主管单位:中华人民共和国农业部
  • 主办单位:中国农业科学院 中国农学会
  • 主编:万建民
  • 地址:北京中关村南大街12号中国农业科学院图书馆楼4101-4103室
  • 邮编:100081
  • 邮箱:zgnykx@caas.cn
  • 电话:010-82109808 82106279
  • 国际标准刊号:ISSN:0578-1752
  • 国内统一刊号:ISSN:11-1328/S
  • 邮发代号:2-138
  • 获奖情况:
  • 中国期刊方阵“双高”期刊,第三届中国出版政府奖提名奖
  • 国内外数据库收录:
  • 美国化学文摘(网络版),英国农业与生物科学研究中心文摘,波兰哥白尼索引,英国动物学记录,日本日本科学技术振兴机构数据库,中国中国科技核心期刊,中国北大核心期刊(2004版),中国北大核心期刊(2008版),中国北大核心期刊(2011版),中国北大核心期刊(2014版),英国食品科技文摘,中国北大核心期刊(2000版)
  • 被引量:85620