中文摘要：

目的探讨空调隔尘网中尘螨过敏原与哮喘发病的关系。方法分别从芜湖市区食堂、商场、酒店和居民家中空调器隔尘网上,及其附近空气中采集灰尘样本各10份。用ELISA法检测粉尘螨1类过敏原（Der f 1）、屋尘螨1类过敏原（Der p 1）的浓度及其灰尘提取液过敏原性。结果芜湖市区食堂、商场、酒店和居民家中空调器隔尘网灰尘中的过敏原Der f 1浓度分别为1.52、1.24、1.31μg/g和1.46μg/g;Der p 1浓度分别约为1.23、1.12、1.16μg/g和1.18μg/g;2种过敏原在开机1 h后的空气中浓度显著高于开机前（P〈0.05）。ELISA分析结果显示,灰尘提取液中过敏原能与尘螨过敏性哮喘小鼠的Ig E抗体产生结合反应。结论芜湖地区居民空调器隔尘网中含有尘螨1类过敏原,可诱发哮喘。

英文摘要：

Objective To discuss the relation between the dust mite allergen（Der）in air-conditioner filters and the asthma attack. Methods The dust samples were collected from the filters of air-conditioners in dining rooms,shopping malls,hotels and households,respectively. The concentrations of Der f 1 and Der p1 were detected by ELISA,and the dust mite immune activities were determined by dot-ELISA. Results The concentrations of Der f 1 in the dining rooms,shopping malls,hotels and households were 1.52,1.24,1.31 μg/g and 1.46 μg/g respectively,and the concentrations of Der p 1 were 1.23,1.12,1.16 μg/g and 1.18 μg/g,respectively. One hour after the running of air-conditioners,the concentrations of Der f 1 and Der p 1 in the air were higher than those before the running of air-conditioners,and the differences were significant（P 〈0.05）. Additionally,the dot-ELISA results revealed that the allergen extracted from the dust was capable of reacting with Ig E from the sera of asthma mice allergic to dust mites. Conclusion Der f 1 and Der p 1 appear abundantly in the filters of air-conditioners in domestic houses in Wuhu City,and the allergens can induce asthma.