中文摘要：

目的：探讨建立痛风性关节炎湿热证病证结合大鼠模型的方法.方法：将40只成年健康雄性SD大鼠随机分为4组,每组10只,体质量（200±20）g.A、B组正常喂养,B组于造模开始后第13天在右侧踝关节腔注射微晶尿酸钠溶液.C、D组正常喂养,同时自由饮用200g· L-1蜂蜜水,并分别以油脂（1 g· 100g-1）或52％红星二锅头酒（1 mL· 100g-1）灌胃,二者交替进行,持续10 d.自第11天开始,置入温度（32±2）℃、相对湿度（92±3）％的人工气候箱中喂养.D组大鼠同时于第13天在右侧踝关节腔注射微晶尿酸钠溶液.造模期间观察大鼠的饮食、精神状态、大小便、舌象等情况;造模开始后第15天,测定尿液水通道蛋白2含量;造模开始后第16天,测定血浆内皮素和血浆降钙素基因相关肽含量;造模结束后处死大鼠,观察舌组织和右侧踝关节滑膜组织的病理变化.结果：①一般情况观察结果.A组大鼠喂养期间,饮食、饮水、活动及大便均正常.B组大鼠造模前期饮食、饮水、活动及大便均正常,自第13天开始饮食、饮水减少,烦躁不安,右踝关节明显增粗,且红、肿、热,伴活动受限.C组大鼠开始造模后逐渐出现饮食、饮水量减少,嗜卧懒动、行动呆滞,毛发蓬松,颜色枯槁,小便黄;造模第5天,开始出现大便溏泻或粘腻;第11天出现明显呼吸粗重,烦躁不安,毛发疏松、粗糙,阴囊松弛下垂,大便溏泻或粘腻症状加重,渐见肛周污秽.D组大鼠造模后症状与C组相同,第13天开始合并出现B组造模后的相同表现.②尿液水通道蛋白2含量测定结果.4组大鼠尿液水通道蛋白2含量比较,差异有统计学意义[（0.251±0.018）mg·mL-1,（0.249±0.020） mg·mL-,（0.233±0.014）mg·mL-1,（0.233±0.011）mg·mL-,F=0.511,P=0.003].A组与B组比较,差异无统计学意义（P=0.760）;A组高于C组和D组（P =0.003;P=0.003）;B组高于C组和D组（P =0.003;P=0.003）;C组和D组比较,差异无统计?

英文摘要：

Objective：To explore the method of building a rat model of gouty arthritis combined with dampness-heat syndrome. Methods：Forty adult healthy male SD rats （weight 200 ＋/-20 g）were randomly divided into 4 groups, 10 rats in each group. Rats in group A and B were fed normally, and rats in group B were administered with intra-articular injection of microcrystalline sodium urate （MSU）solu- tion in the right ankle on the 13th day after the beginning of modeling. Rats in group C and D were fed normally. Meanwhile,they could drink the honey water（200 g/L）freely and they were intragastric administrated with grease （1 g/100 g）and Chinese spirits （52% （vol/ vol）, 1 mL/100 g）in turn for 10 consecutive days. Since the 11 th day, they were fed in the artificial climate box （temperature,32 ＋/-2 de- grees centigrade;relative humidity,92 ＋/-3 % ）. Meanwhile, the rats in group D were administered with intra - articular injection of MSU solution in the right ankle on the 13th day. The rats＇ s diet, mental status,urination and defecation and tongue demonstration were observed during modeling period. The urine contents of aquaporin- 2（ AQP2 ）were measured on the 15th day from the beginning of modeling. The plasma contents of endothelin（ET） and calcitonin generelated peptide（CGRP） were measured on the 16th day from the beginning of model- ing. All rats were executed after the modeling and the pathological changes in tongue tissue and synovial tissue of right ankle joint were ob- served. Results ： During feeding period, eating, drinking, activities and excrements of the rats in group A were normal. Eating, drinking, activ- ities and excrements of the rats in group B were normal in the early period after the beginning of the modeling,while since the 13th day, their eating and drinking began to decrease and they became restless. Thickening, red, swelling, hot and action-limited were found in the right ankle joints. After the beginning of modeling, rats in group C presented with reduct