目的:建立一种同时测定大枣中齐墩果酸和白桦脂酸的方法。方法:采用HPLC法,色谱柱为HyperilBDSC18(4.6mm×250mm,5滋m),甲醇颐水(85颐15)为流动相,检测波长为210nm,柱温25℃。结果:齐墩果酸、白桦脂酸在0.008耀0.200mg/mL呈良好的线性关系,平均加样回收率分别为101.25%,101.64%,RSD分别为2.44%,1.25%。结论:该方法操作简单、准确、重复性好,可用于大枣的质量控制。
Objective:To establish the methods for measuring oleanolic acid and betulinic acid in jujube simultaneously.Methods:HPLCwas adpoted,chromatographic column was Hyperil BDSC18(4.6mm250mm5m),ratio between methyl alcohol and water(8515)was moving phase,detection wavelength was210nm,column temperature was25℃.Results:Oleanolic acid and betulinic acid had good linear relation from0.008to0.200mg/mL,average recycle rate101.25%and101.64%seperately,RSD were2.44%and1.25%seperately.Conclusion:This method is simple accurate and reproducible,which can be used for quality control of jujube.