研究了料液比、超声时间、超声温度及超声功率对蜜环菌出油率的影响,并通过正交试验确定了提取蜜环菌油最佳工艺。结果表明:最佳提取工艺是料液比(g∶m L)1∶4,时间30 min,温度40℃,功率400 W。应用气相色谱-质谱联用技术研究了野生蜜环菌子实体中油类成分及其相对含量,确认其中9个成分,分别为亚油酸、硬脂酸、软脂酸、羊脂酸、正壬酸、月桂酸、肉豆蒄酸、正十五烷酸和反式棕榈油酸。
The efficiency of oil extraction from Armillaria mellea were studied by adjusting the solid and liquid ratio,the ultrasonic time,the ultrasonic temperature and the ultrasonic wave power. The results of orthogonal test showed that the optimal conditions of ultrasonic extraction of oil from A.mellea with include the solid and liquid ratio( g ∶ m L) 1 ∶ 4,ultrasonic time for 30 min,ultrasonic temperature at 40℃ and ultrasonic wave power 400 W. The chemical constituent of oil from fruiting body of Armillaria mellea was firstly analyzed with GC-Ms,and its contents of 13 compounds were identified to be linolic acid,stearic acid,palmitic acid,caprylic acid,nonanoic acid,lauric acid,tetradecanoic acid,palmitelaidic acid.