盔孢伞属Galerina隶属于担子菌门Basidomycota蘑菇纲Agaricomycetes蘑菇目Agaricales球盖菇科Strophariaceae,目前该属全球己知300多种,多分布于北半球。中国报道30余种,目前20余种己得到确认,本文是对以往工作的补充。在对该属新近采集的标本进行研究时,发现3个中国新记录种,即小盔孢伞G.pumila、阿氏盔孢伞G.atkinsoniana和纹柄盔孢伞G.stylifera,对其进行详细的形态特征描述,并绘制其担子体宏观结构及光学显微镜下其担孢子、担子、缘生囊状体、柄生囊状体、菌盖表皮菌丝显微结构的线条图,提供了扫描电子显微镜的担孢子照片,对所研究材料进行DNA提取、PCR扩增、测序获得其ITS序列并上传到GenBank。下载GenBank中通过Blast比对所得该属北美序列及对该属分子系统学文献中的盔孢伞属欧洲序列,选择盔孢伞属的姊妹群裸伞属Gymnopilus作为外类群,并与所用材料获得的ITS序列结合,通过贝叶斯建立系统发育树。从系统发育树结果看,本文报道的3个新记录种皆与所下载的该种序列聚在一枝,且支持率较高,验证了3个新记录种的形态学鉴定结果。
The basidiomycete genus Galerina (Basidomycota, Agaricomycetes, Agaricales, Strophariaceae) accommodates more than 300 species worldwide, predominantly described from the northern hemisphere, and more than 30 species were found in China, of which more than 20 species have been confirmed; this paper is a supplement to previous work. Three species of the genus 6alerina, namely G. pumila, G. atkinsoniana and G. stylifera were reported from China for the first time. The morphological descriptions are given in detail. The line drawings of the macrostructures of basidiomata and microstructures of basidiospores, basidia, cheilocystidia, caulocysUdia and pileipellis are provided. SEM images of the ornaments on the surface of basidiospores are given. ITS sequences obtained by the authors and some sequences obtained from EMBL/GenBank are analyzed to affirm the reseults of previous morphological identifications. GenBank sequences of Gymnopilus junonius and Gymnopilus sepineus are used as outgroup in the analyses of ITS. Bayesian phylogenetic tree strongly affirmed the identity of morphologica dentification of the three Galerina species