从喜树Camptotheca acuminate树皮和果实中分离得到27株内生真菌,发酵后经HPLC检测,筛选出一株菌丝产喜树碱的菌,产量达774μg/L。对其ITS序列进行系统发育分析,结合其培养特征和显微特征,鉴定为拟茎点霉属(Phomopsis sp.)。这是首次报道分离自喜树的该属真菌发酵产喜树碱。
27 endophytic fungi were isolated from the barks and fruits of Camptotheca acuminate and then cultured in submerged fermentation.The fermentation products of each strain were analyzed with high performance liquid chromatography(HPLC) which showed that one produced camptothecin with the output of 774 μg/L.The strain was identified as Phomopsis sp.by phylogenetic analysis based on ITS rDNA and cultural and morphological characteristics.This was the first report that Phomopsis sp.,which was isolated from Camptotheca acuminate,produced camptothecin.