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中文摘要：
目的建立一测多评法同时测定毛冬青配方颗粒中6种酚酸类成分的含量。方法以绿原酸为内参物,分别建立其与隐绿原酸、咖啡酸、异绿原酸B、异绿原酸A、异绿原酸C的相对校正因子,并利用该相对校正因子计算含量;同时利用外标法测定该6种酚酸的含量,比较2种测定方法的差异,验证一测多评法的准确性和可行性。结果相对校正因子的重现性良好,8批毛冬青配方颗粒的计算值与实测值无显著性差异。结论该方法可用于毛冬青配方颗粒中多指标成分质量评价。
英文摘要：
OBJECTIVE To establish a method for simultaneous detemination of six phenolic acids in Ilex pubescens formula granules by quantitative analysis of multi-components by single marker（QAMS）. METHODS Chlorogenic acid was used as the internal reference substance. The relative correction factors（RCF） of chlorogenic acid, cryptochlorogenic acid, caffeic acid, isochlorogenic acid B, isochlorogenic acid A, isochlorogenic acid C were established respectively, and their contents in samples were calculated by RCF. At the same time, the external standard method（ESM） was used to detemine the above six phenolic acids. Compared with the content results determined by the ESM and QAMS, the accuracy and feasibility of QAMS method were verified. RESULTS The reproducibility of RCF was acceptable. There was no significant difference between the quantitative results of the above two methods in eight batches of Ilex pubescens formula granules. CONCLUSION The method can be used as quality evaluation method of multi-component in Ilex pubescens formula granules.
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