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中文摘要：
[目的]研究T-2毒素对人结肠癌细胞SW115的增殖抑制与凋亡作用。[方法]体外培养人结肠癌细胞SW115,加入浓度为1.6、8、40、200、1 000μg/m L的T-2毒素作用24 h,MTT法检测细胞增殖抑制率,流式细胞仪检测细胞凋亡,Hoechest 33258染色法观察细胞凋亡形态,并检测Caspase-3的活性变化。[结果]与对照组相比,T-2毒素为40μg/m L时显著抑制SW115细胞增殖（P〈0.01）,当T-2毒素达到200μg/m L,流式细胞仪检测凋亡率为27.55%,细胞内Caspase-3活性为0.597,差异具有统计学意义（P〈0.01）。[结论]T-2毒素对人结肠癌细胞SW115具有明显的抑制作用,具有诱导SW115细胞凋亡的作用。
英文摘要：
[ Objective] Inhibitory and apoptosis effects was investigated with T- 2 toxin on human colon cancer SWl15. [ Methods] The SW115 cells cultured in vitro cells were incubated with T- 2 toxin at the concentration of 1.6,8,40,200 and 1 000 μg/mL for 24 hours. The proliferation of SW115 were examined by MTT cycle and apoptosis analysed by flow cytom- ctry, Hochest 33258 staining was used to observe of cell morphology, the activity change of Caspase -3 were detected. [ Re- suits] Compared with the normal control group, the proliferation of SW115 was significantly inhibited by T -2 toxin when the concentration of 40 μg/mL（ P 〈 0.01 ）. The apoptosis rate was 27.55 % by flowcytometry （FCM） when T -2 toxin reached 200 μg/mL, and the Caspase -3 activity was increased 0. 597 which was significantly different from the control group （P 〈 0.01 ）. [ Conclusion] Human colon cancer SW115 cells was significantly inhibited and induced apoptosis by T- 2 toxin.
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