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中文摘要：
以新疆野苹果（Malus sieversii（Lebed.）M．Roem.）无菌试管苗为试材，对其离体茎尖玻璃化超低温保存的影响因素进行研究。结果表明，新疆野苹果茎尖在含有5％二甲基亚砜（DMSO）的0．4mol／L蔗糖培养基上预培养3d，60％玻璃化溶液（PVS2）中室温装载30min，PVS：0℃下处理40min，经液氮保存至少24h后，转入继代培养基上再培养，成活率和再生率分别为93．3％和86．7％。再生植株生长和分化正常；同时对再生植株进行SSR标记检测，未发现超低温保存前后的DNA谱带存在差异。
英文摘要：
Experiments were conducted about tissue culture systems and cryopreservation on Xinjiang wild apple（Malus sieversii （Lebed.） M.Roem.）. A suitable procedure was established as follows： four weeks after subculture, shoot-tips about 2 - 3 cm in length were precultured for 3 days on MS medium supplemented with 0. 4 mol /L sucroseand 5% DMSO, and pretreated in 60% plant vitrification solution （PVS2） for 30 minutes at room temperature, then followed by dehydration with PVS2 for 40 minutes at O℃ and put into liquid nitrogen for 24 hours. The survival and regeneration rate of shoot tips treated in this way was 93.3% and 86.7% respectively. The plantlets could nomally grow and differentiation. The plantlets were subjected to genetic analysis using SSR molecular marker, and the genetic difference was not found.
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