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中文摘要：
目的：研究跨膜激活剂及钙调亲环素配体相互作用分子的免疫球蛋白（transmembrane activator or calcium-modulating cyclophylin ligand-interactor-immunoglobulin,TACI-Ig）对rh BLy S刺激佐剂性关节炎（adjuvant arthritis,AA）大鼠肠系膜淋巴结细胞（mesenteric lymph node lymphocytes,MLNLs）功能的影响。方法：rh BLy S刺激AA大鼠MLNLs,设TACI-Ig五个浓度给药组（0.01,0.1,1.0,10,100μg/m L）和一个阴性对照组Ig G-Fc（10μg/m L）。MTT法测MLNLs增殖;流式细胞仪测T细胞主要亚群变化;ELISA法检测IL-17水平;Western blot法测TACI蛋白表达。结果：TACI-Ig降低AA大鼠MLNLs增殖能力;降低CD3^＋/CD4^＋和CD4^＋/CD25^＋T细胞,升高CD4＋/CD62L＋T细胞的百分含量;降低AA大鼠MLNLs中IL-17水平和TACI蛋白表达。结论：TACI-Ig对MLNLs增殖活化、细胞因子分泌及受体蛋白表达均有抑制作用。
英文摘要：
AIM： To investigate the TACI-Ig on rh BLys stimulated MLNLs from AA rats. METHODS： MLNLs from AA rats were stimulated by rhBLy S in vitro. Set five concentrations of TACI-Ig（ 0. 01,0. 1,1. 0,10,100 μg /m L） groups and a negative control group Ig G Fc（ 10 μg / m L）. Proliferations of MLNLs were assayed by MTT. The proportions and activity of T cell in MLNLs were analyzed by flow cytometry. The levels of IL-17 were measured by ELISA. The expressions of TACI were investigated by western blotting. RESULTS： TACIIg inhibit the proliferative reactions of MLNLs from AA rats. And TACI-Ig decreased the CD3^＋CD4^＋T cell and CD4^＋CD25^＋T cell,increased the CD4^＋CD62L＋T cell. Also, TACI-Ig decreased IL-17 production and TACI expression. CONCLUSION：TACI-Ig has inhibitory action on cell proliferation,cytokines secretion and receptor protein expression of MLNLs.
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