欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
目的研究川芎嗪抗肿瘤转移作用与改善肿瘤介导的高凝状态两者之间的相关性。方法采用C57BL6小鼠的实验性转移模型,考察川芎嗪体内对肿瘤血行转移的影响,通过血液APTT、PT、TT、FIB检测考察肿瘤对凝血时间的影响及药物对其的改善作用,同时通过ELISA方法检测血浆t-PA、u-PA、PAI反映纤溶系统的活性变化,测定血浆中纤维蛋白肽A反映纤维蛋白的生成情况,检测血浆中FXⅢ来反映纤维蛋白的稳定程度。结果体内川芎嗪72 mg·kg-1能够抑制B16F10实验转移,明显减少肺转移结节数量。实验性转移模型组与正常组相比,凝血时间明显缩短,表现出血液高凝状态,给予川芎嗪8、24、72 mg·kg-1,不同时间均可以不同程度的延长APTT、PT、TT、FIB,并能抑制纤维蛋白的形成和稳定,进而改善肿瘤引起的血液高凝。结论肿瘤转移会引起血液的高凝,且随着转移时间的延长,血液高凝程度加剧。川芎嗪能够改善肿瘤介导的高凝状态,可能与其抗肿瘤转移作用相关。
英文摘要：
Aim To study the association between antimetastasis and improvement of tumor-mediated hypercoagulability of Ligustrazine of the main component of traditional Chinese medicine Chuanxiong.Methods The experimental metastasis model was used to study the overall efficacy of anti-metastasis of Ligustrazine.The APTT,PT,TT,FIB were tested to evaluate the effect of tumor on coagulation system and improvement of Ligustrazine.The change in the activity of fibrinolytic system was detected plasma level of t-PA,u-PA,PAI by Elisa kit.Fibrin formation was assessed by detecting plasma fibrinopeptide A（FPA） by FPA Elisa kit.The FXIII Elisa Kit was used to detect the stability of fibrin.Results Ligustrazine 72 mg·kg-1 inhibited B16F10 experimental metastasis in vivo,a significant reduction in the number of lung nodules.Clotting time of experimental metastasis model group was significantly reduced compared with normal group,showing hypercoagulability.The coagulation time of APTT,PT,TT,FIB was extended to some different extent after treatment by Ligustrazine 8 mg·kg-1,24 mg·kg-1,72 mg·kg-1 at 7,14,21th day.Besides,Ligustrazine could inhibit fibrin formation and stability,thereby improving tumor-induced hypercoagulability.Conclusions Tumor metastasis can cause blood coagulation and activate blood system,and with the transfer time extend,the hypercoagulability is more serious.Ligustrazine can inhibit tumor metastasis which may be related to the improvement of the tumor-mediated hypercoagulable state.
同期刊论文项目
同项目期刊论文
Bioactive tanshinones in Salvia miltiorrhiza inhibit the growth of prostate cancer cells in vitro an
Cryptotanshinone has diverse effects on cell cycle events in melanoma cell lines with different meta
Xanthatin induces cell cycle arrest at G2/M checkpoint and apoptosis via disrupting NF-κB pathway in
Comparative Effects of Hirudo, Frankincense, and Motherwort on Inhibition of Blood Platelet Aggregat
Luteolin reduces the invasive potential of malignant melanoma cells by targeting β3 integrin and the
Inhibition of hypoxia-induced epithelial mesenchymal transition by luteolin in non-small cell lung c
Effect of Aqueous Extracts of Several Kinds of Herbs on Human Platelet Aggregation and Expression of
Chemomodulatory effi cacy of lycopene on antioxidant enzymes and carcinogen-induced cutaneum carcino
Holothurian glycosaminoglycan inhibits metastasis and thrombosis via targeting of nuclear factor-kap
Inhibition of vascular endothelial growth factor-mediated angiogenesis involved in reproductive toxi
Holothurian Glycosaminoglycan Inhibits Metastasis and Thrombosis viaTargeting of Nuclear Factor-κB/T
Concerted Suppressionof STAT3 and GSK3β Is Involved in Growth Inhibition of Non-Small Cell LungCance
CryptotanshinoneActivates p38/JNK and Inhibits Erk1/2 Leading to Caspase-Independent Cell Deathin Tu
Luteolin reduces theinvasive potential of malignant melanoma cells by targeting β3 integrin and thee
Inhibition of hypoxia-induced epithelialmesenchymal transition by luteolin in non-small cell lung ca
The Angiogenic Responses Induced by Release of AngiogenicProteins from Tumor Cell-Activated Platelet
Cryptotanshinone, a novel tumor angiogenesisinhibitor, destabilizes tumor necrosis factor-a mRNA via
Chemopreventive efficacy of menthol on carcinogen-induced cutaneous carcinoma in mice throughinhibit
Cancer-promotingeffect of capsaicin on DMBA/TPA-induced skin tumorigenesis by modulatinginflammation
Holothurian glycosaminoglycan inhibits metastasis via inhibition of P-selectin in B16F10 melanoma ce
PKM2 Regulates Hepatocellular Carcinoma Cell Epithelial-mesenchymal Transition and Migration upon EG
PKM2 Regulates Hepatocellular Carcinoma Cell Epithelialmesenchymal Transition and Migration upon EGF
Phenolcarboxylic acids from medicinal herbs exert anticancer effects through disruption of COX-2 act
期刊信息
位置：