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中文摘要：
建立福林-酚法测定黑米总酚含量的方法。考察不同影响因素对测定黑米总酚含量的影响,并测定不同产地黑米的总酚含量。结果表明：最佳反应条件为当福林-酚试剂的浓度为0.20 mol/L、Na_2CO_3溶液的质量分数为2.5%、体系反应时间为40 min、反应温度为50℃时,没食子酸标准液在0~12μg/m L范围内,总酚含量与其吸光度值有良好的线性关系。该方法操作方便,精密度高,回收率范围为92.90%~107.25%。不同产地黑米总酚含量在43~66 mg/100g之间,有较大差异。
英文摘要：
A method of determining total phenol content in black rice by Folin-ciocalteu method was established. The effects of different factors on the determination method of total phenol content in black rice were studied. And the total phenol content in different black rices from different areas were detected. The results showed that the optimal conditions were as follows： Folin-ciocalteu concentration 0.20 mol/L, Na_2CO_3 solution 2.5%, reaction time 40 min and reaction temperature 50 ℃.When the standard gallic acid was within 0-12 μg/m L, the total phenol content and absorbance values had a good linear relationship. The method was easy to operate with high precision, and the recycle range was from 92.90% to 107.25%. The total phenol contents of black rices from different areas had a bigger difference. It was in the range of 43-66 mg/100 g.
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