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寒露林蛙的核型和Ag-NORs研究
  • 期刊名称:四川动物
  • 时间:0
  • 页码:704-706
  • 语言:中文
  • 分类:Q959.5[生物学—动物学]
  • 作者机构:[1]沈阳师范大学中国特有两栖爬行动物繁育和保护研究中心,辽宁省生物进化与生物多样性重点实验室,沈阳110034
  • 相关基金:本研究得到国家自然科学基金(No:30470206,3087070276)的资助 致谢:感谢廉静和李晶尧同学帮助采集标本!
  • 相关项目:中国的林蛙及趾沟蛙的分类研究
中文摘要:

采用常规骨髓细胞制片法对产于湖南省双牌县的寒露林蛙的核型及NORs位点进行了观察和分析。结果表明寒露林蛙与分布于我国南方的其他林蛙相同:2n=26,NF=52,为5+8核型模式。寒露林蛙核型为14M+10SM+2ST,无次溢痕,NORs位于第11对染色体长臂的中部。通过比较分析我国2n=26的6种林蛙的核型等细胞遗传学信息,本文结果支持形态分类和分子系统发生的研究结论,认为寒露林蛙是一有效种。

英文摘要:

In this work, we present for the first time cytogenetic data of the Hanlu brown frog (Rana hanluica Shen et al., 2007 ) collected from the type locality, Shuangpai County, Hunan Province, southern China. The karyotype and NOR local- ities were described and compared with other Chinese brown frogs. Rana hanluica had similar cytogenetic information to the other brown frogs which had 26 chromosomes : 2n = 26 with 5 large-sized pairs and 8 small-sized pairs, and NF = 52. The karyotype of R. hanluica was 14M + 10SM + 2ST, and no secondary constriction was observed. Ag-NORs were located at 1 1qinter. In comparison with other brown frogs from China, the cytogenetic data from R. hanluica supported the taxonomic and molecular phylogenetic analysis for this species and indicated that R. hanluica was a valid species.

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