中文摘要：

同种移植炎症因子AIF-1（allograft inflammatory factor 1,AIF-1）是一种由干扰素γ诱导的含有EF-hand结构域的钙离子结合蛋白,其功能主要是参与移植排斥、免疫炎症反应、自身炎性和非炎性的损伤等。首次克隆了杂色鲍AIF-1基因cDNA全序列,命名为HdAIF-1,其全长为942 bp,开放阅读框为456 bp,编码151个氨基酸。实时荧光定量PCR结果表明：HdAIF-1在杂色鲍各组织中均有表达,其中在血淋巴和鳃中表达量最高。高温应激下,HdAIF-1在鳃组织中各时相表达均显著上调,并在温度升至31℃时达到最高。而血淋巴和肝胰腺中HdAIF-1在高温应激前4个时相表达无显著差异,到了96 h均显著上调。缺氧应激下,HdAIF-1在血淋巴中表达变化没有显著差异,而鳃中24 h显著下调,192 h显著上调。副溶血弧菌感染实验表明HdAIF-1基因在感染后3、24和48 h均检测到HdAIF-1的表达量显著上调。高温和缺氧应激以及弧菌感染均显示HdAIF-1基因表达量发生显著变化,说明HdAIF-1可能作为免疫因子在杂色鲍应激等状况下发挥重要作用。

英文摘要：

Allograft inflammatory factor-1（AIF-1）is a kind of calcium-binding protein with EF-hand domain.AIF-1 mRNA is robustly induced by IFN-γ in murine macrophages.It is involved in transplant rejection,immune inflammatory reaction,non-inflammatory injury and so on.In this study,a molluscan AIF-1 gene,HdAIF-1,was cloned for the first time from Haliotis diversicolor.Its full-length cDNA sequence is 942 bp,with a 456 bp open reading frame encoding a protein of 151 aa.Quantitative real-time PCR results indicated that HdAIF-1 could be detected in all examined tissues,with the highest level in hemolymph and gill.Under thermal stress,HdAIF-1 was up-regulated in gill significantly at temperature-rise period and achieved the highest level when up to 31 ℃.However,the expression level of HdAIF-1 in hemolymph and hepatopancreas did not show significant difference between thermal group and control group from the first phase to the fourth,and its expression level was up-regulated significantly at 96 h in these two tissues.Under hypoxia stress,the expression level of HdAIF-1 in hemolymph showed no significant difference between control and exposed groups.However,it was down-regulated at 24 h and up-regulated at 192 h remarkably in gill.After Vibrio parahaemolyticus challenge,the HdAIF-1 expression level achieved the highest level at 3 h in hemolymph,reduced to the control level at 12 h,and then up-regulated significantly at both 24 h and 48 h.These results suggest that HdAIF-1 may play important roles as an immune factor under different stresses.