中文摘要：

碱性磷酸酶（alkaline phosphatase,ALP）是一种广泛分布的单脂磷酸水解酶,是动物生物代谢过程中重要的调控酶。实验首次克隆了大黄鱼ALP基因cDNA全序列,命名为LcALP,其全长为2 345 bp,开放阅读框为1 629 bp,编码543个氨基酸。实时荧光定量PCR检测发现,LcALP在大黄鱼雌雄各组织中均有表达,其中在眼和头肾的表达量最高,雌性鳃和脑中LcALP表达量显著高于雄性,而在性腺和脾脏中的表达量则显著低于雄性;胚胎发育过程中,多细胞期、囊胚期和原肠期LcALP基因表达水平相对较低,在卵黄栓形成期明显提高,至孵出期达到峰值,而初孵仔鱼期则显著下调;大黄鱼肌肉细胞系经LPS处理后6 h LcALP表达量降低,12 h显著下调,而poly I：C处理后表达水平持续上升,24 h显著上调至峰值。研究表明,LcALP在大黄鱼胚胎发育调控以及机体免疫防御中发挥重要作用。

英文摘要：

Alkaline phosphatase（ ALP） is a widely-distributed phylogenetic phosphomonoesterase involved in the precise regulation of the animal biological metabolism. In this study,an ALP gene,LcALP,was cloned for the first time from Larimichthys crocea. Its full-length cDNA sequence is 2 345 bp,with a 1 629 bp open reading frame encoding a protein of 543 aa. qRT-PCR revealed the expression of LcALP could be detected in all adult tissues examined,with higher expression in the eye and head kidney of both sexes. Compared with the male,the expression level of LcALP gene from female was significantly higher in the gill and brain whereas much lower in the gonad and spleen. The low levels of LcALP were observed during the multiple cells,blastula,and gastrula stages. The levels increased significantly at yolk plug stage and peaked at prehatching stage,and then declined obviously at the alevin stage. After treatment with LPS,the expression level of LcALP in muscle cell line began to decrease at 6 h and was down-regulated significantly at 12 h.When large yellow croaker muscle cell lines were treated with poly I： C,the expression level of LcALP continuously increased to the peak at 24 h. Our results suggested that LcALP might play important roles as a regulator during the embryonic development and as an immune factor under stimulation of different pathogen-associated molecular patterns.