中文摘要：

酪氨酸3-加单氧酶／色氨酸5-加单氧酶激活蛋白ξ（14—3—3ξ蛋白）是14—3-3蛋白家族的亚型之一。14—3—3蛋白是一种高度保守的酸性可溶性蛋白，它可以与激酶、磷酸酶等多种信号蛋白结合，并在信号转导和细胞凋亡等过程中发挥相应的作用。本实验通过SMART—RACE技术首次获得了杂色鲍14—3—3ξ基因的全长，并命名为Hd 14—3—3ξ，其cDNA全长为3010bp，ORF为819bp，共编码272个氨基酸。实时荧光定量PCR结果显示，Hd14—3—3ξ基因在杂色鲍各组织中均有表达，其中在肝胰腺和血细胞中的表达量相对较高。当处于缺氧环境下时，鳃组织中Hd14—3—3ξ的表达在4h时存在显著差异，血细胞中Hd14—3—3ξ在24h和96h时表达量显著上调。高温应激下，Hd14—3—3ξ在血细胞中的表达量无显著变化，而鳃组织中Hd14—3—3ξ在96h的表达量却显著上调。高温缺氧联合应激实验表明，鳃组织中Hd14—3—3ξ在4h、24h和96h均显著上调，而血细胞中的表达量没有显著性变化。经检测发现，副溶血弧菌注射后，鳃组织中Hd14—3-3ξ在3h和24h，血细胞中在6h和24h的表达量显著上调。以上温度、溶氧量等环境因素以及病原菌的刺激均会导致Hd14—3—3ξ在不同组织中表达量的显著变化，说明Hd14-3-3ξ在杂色鲍的免疫反应中可能扮演着重要的角色。

英文摘要：

Abstract ： Tyrosine-3 -monooxygenase/Tryp-tophane-5-monooxygenase activator protein （ 14-3-3 ξ protein ） is one of the isoform of 14-3-3 proteins which are highly conserved small acidic proteins. Several studies have shown that 14-3-3 proteins can bind many signal proteins, such as kinase and phosphatase, and play crucial roles in many biological processes including regulation of signaling and cell apoptosis. In this study,the full length cDNA of 14-3-3ξ from Haliotis diversicolor was cloned for the first time by SMART-RACE and named Hd14-3-3ξ . Its full-length cDNA sequence was 3 010 bp, with an open reading frame of 819 bp encoding a protein of 272 amino acids. The Real-time Quantitative PCR results showed that Hd14-3-3ξ was detected in all examined tissues and with the highest expression level in hepatopancreas and haemocytes. When under the stress of hypoxia, the expression level of Hd14-3-3ξ in haemocytes of exposed groups showed a significant up-regulation at 4 h,but in gill it happened at 24 h and 96 h. Under thermal stress,in haemocytes,it showed no significant difference between control and thermal groups but it was up-regulated significantly at 96 h in gill. Under thermal ＆ hypoxia stresses,it showed no significant difference between control and exposed groups in haemocytes. However,in gill,it was up-regulated significantly at 4 h,24 h and 96 h. After Vibrio parahaemolyticus challenge, the expression of Hd14-3-3ξ reached a significant level at 6 h in haemocytes and reduced to the normal level at 12 h, and then reached the highest significant level at 24 h. In gill,the significant expression level appeared at 3 h and 24 h and there was no difference between other time phases. All these results indicate that Hd14-3-3ξ may play important roles in immunoreaction of H. diversicolor.