欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
硝基还原酶是一类依赖于黄素单核苷酸或黄素腺嘌呤二核苷酸的细胞质酶,广泛存在于细菌中。肿瘤细胞缺氧通常也可导致胞内硝基还原酶增加。在电子供体如还原型烟酰胺腺嘌呤二核苷酸的存在下,硝基还原酶可以将芳香族硝基化合物有效还原为相应的氨基化合物。这种还原行为不仅用于药物的激活和芳香族硝基化合物的生物降解,同时也可用来设计含硝基的荧光探针对实体瘤细胞的缺氧状况进行检测。本文简要评述近年来硝基还原酶荧光探针的研究进展,包括基于多米诺分解反应以及荧光体上硝基直接还原为氨的反应而发展的荧光探针。
英文摘要：
The nitroreductase family comprises a group of flavin mononucleotide or flavin adenine dinucleotide dependent enzymes.These enzymes can be found in bacterial species and can effectively reduce nitroaromatic compounds to the corresponding amines in the presence of nicotinamide adenine dinucleotide or nicotinamide adenine dinucleotide phosphate as an electron donor.This behavior has been widely used not only in eliminating pervasive nitroaromatic pollutants and activating nitrofuran antibiotics,but also in developing new fluorescent probes for the detection of nitroreductase and hypoxia in some biosystems such as tumor cells.In this paper,we briefly reviewed the recent advances in nitroreductase fluorescent probes and their analytical applications.Two major design strategies for nitroreductase probes have been summarized,which are based on the domino reaction and direct reduction of nitro groups on the fluorochromes to the corresponding amino groups.
同期刊论文项目
同项目期刊论文
A Cresyl Violet-Based Fluorescent Off-On Probe for the Detection and Imaging of Hypoxia and Nitrored
Sensitive and Selective Near-Infrared Fluorescent Off-On Probe and Its Application to Imaging Differ
Sensitive detection of ozone by a practical resorufin-based spectroscopic probe with extremely low b
3,4-Dinitrobenzamide Functionalized CdTe/ZnTe Quantum Dots as a Nanoprobe for Imaging Glutathione S-
Assay of vitamin B in urine by capillary electrochromatography with methacrylate-based monolithic co
Parallel comparative studies on the toxic effects of unmodified CdTe quantum dots, gold nanoparticle
A spectroscopic off-on probe for simple and sensitive detection of carboxylesterase activity and its
A novel chiral ligand exchange capillary electrophoresis system with amino acid ionic liquid as liga
Imaging Different Interactions of Mercury and Silver with Live Cells by a Designed Fluorescence Prob
A simple and sensitive method for visual detection of phosgene based on the aggregation of gold nano
Magnetically separable Fe3O4-Ag3PO4 sub-micrometre composite: facile synthesis, high visible light-d
Poly(m-phenylenediamine)-Based Fluorescent Nanoprobe for Ultrasensitive Detection of Matrix Metallop
7-((5-Nitrothiophen-2-yl)methoxy)-3H-phenoxazin-3-one as a spectroscopic off-on probe for highly sen
Charge-transfer interaction between melamine and quinones: Towards voltammetric determination of mel
In situ formation of three-dimensional and uniform Pt/Carbon nanotube nanocomposites from ionic liqu
Microchip CE-LIF method for the hydrolysis of L-glutamine by using L-asparaginase enzyme reactor bas
Cysteine-modulated colorimetric sensing of extracellular Mg2+ in rat brain based on the strong chela
Real-time colorimetric assay of inorganic pyrophosphatase activity based on reversibly competitive c
ZeoliticImidazolate framework-based electrochemical biosensor for in vivo electrochemical measuremen
Microfluidic chip-based online electrochemical detecting system for continuous and simultaneous moni
Photodecomposition of Ferrocenedicarboxylic Acid in Methanol to Form an Electroactive Infinite Coord
Folic acid-functionalized fluorescent gold nanoclusters with polymers as linkers for cancer cell ima
Hybridization of Bioelectrochemically Functional Infinite Coordination Polymer Nanoparticles with Ca
Immobilization of trypsin via reactive polymer grafting from magnetic nanoparticles for microwave-as
Competitive Coordination of Cu2+ between Cysteine and Pyrophosphate Ion: Toward Sensitive and Select
Bioelectrochemically Active Infinite Coordination Polymer Nanoparticles: One-Pot Synthesis and Biose
Sensitive and selective voltammetric measurement of Hg2+ by rational covalent functionalization of g
Study on the decrease of renal D-amino acid oxidase activity in the rat after renal ischemia by chir
A graphene oxide-peptide fluorescence sensor tailor-made for simple and sensitive detection of matri
Rhodamine B Piperazinoacetohydrazine: A Water-Soluble Spectroscopic Reagent for Pyruvic Acid Labelin
Use of a rhodamine-based bifunctional probe in N-terminal specific labeling of Thermomyces lanuginos
A highly specific ferrocene-based fluorescent probe for hypochlorous acid and its application to cel
Determination of sodium benzoate by chiral ligand exchange CE based on its inhibitory activity in D-
Online Electrochemical Measurements of Ca2+ and Mg2+ in Rat Brain Based on Divalent Cation Enhanceme
A non-oxidative electrochemical approach to online measurements of dopamine release through laccase-
Potential-controllable green synthesis and deposition of metal nanoparticles with electrochemical me
Spectroscopic Response of Ferrocene Derivatives Bearing a BODIPY Moiety to Water: A New Dissociation
An unprecedented strategy for selective and sensitive fluorescence detection of nitric oxide based o
A new resorufin-based spectroscopic probe for simple and sensitive detection of benzoyl peroxide via
Reactive polymer as a versatile toolbox for construction of multifunctional superparamagnetic nanoco
Amphiphilic block copolymer modified magnetic nanoparticles for microwave-assisted extraction of pol
A versatile method for the preparation of polyacrylamide derivative functionalized thermo-responsive
Preparation of poly(N-isopropylacrylamide)-grafted well-controlled 3D skeletal monolith based on E-5
A colorimetric sensor for determination of cysteine by carboxymethyl cellulose-functionalized gold n
A chiral ligand exchange CE essay with zinc(II)-L-valine complex for determining enzyme kinetic cons
Distinguishing Folate-Receptor-Positive Cells from Folate-Receptor-Negative Cells Using a Fluorescen
Fluorescence sensing of adenosine deaminase based on adenosine induced self-assembly of aptamer stru
Nitroreductase Detection and Hypoxic Tumor Cell Imaging by a Designed Sensitive and Selective Fluore
Alkaline Post-Treatment of Cd(II)-Glutathione Coordination Polymers: Toward Green Synthesis of Water
Facile one-pot synthesis of L-proline-stabilized fluorescent gold nanoclusters and its application a
Monolith and coating enzymatic microreactors of L-asparaginase: kinetics study by MCE-LIF for potent
In vivo electrochemical monitoring of the change of cochlear perilymph ascorbate during salicylate-i
期刊信息
位置：