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中文摘要：
在生活房间学习 mRNA 的表示水平将在房间生物学研究，疾病诊断，和药发现提供巨大的晋升机会。在这篇论文，分子的烽火(MB ) 为重要肿瘤特定压制或基因 p21 被设计了并且综合。荧光信号被检测在在进入的 MB 以后即时鼻咽的癌房间的细胞质。在把 p21MB 注入鼻咽的癌房间和 p33-transfected 以后鼻咽的癌房间，荧光灯的信号紧张的一致增加在房间线，和在大约 15 min 完成的最大的荧光紧张被检测。在跟随微注射的大约 4 min，增加率的荧光在这二根房间线之间是显著地不同的，它显示不同 p21 mRNA 表示层次。在即时察觉获得的结果被 RT-PCR 也验证。增加率的起始的荧光的分析能高效地减少酶的副作用并且在生活房间 mRNA 察觉改进精确性。
英文摘要：
Studying the expression level of mRNA in living cells will offer tremendous opportunities for advancement in cell biology research, disease diagnostics, and drug discovery. In this paper, a molecular beacon （MB） specific for the important tumor suppressor gene p21 has been designed and synthesized. The fluorescence signal was detected in real-time after the MB entered the cytoplasm of nasopharyngeal carcinoma cells. After injecting the p21MB into nasopharyngeal carcinoma cell and p33-transfected nasopharyngeal carcinoma cell, the consistent increase of fluorescent signal intensity was detected in both cell lines, and maximum fluorescence intensity achieved in about 15 min. In about 4 min following microinjection, the fluorescence increasing rate was significantly different between these two cell lines, which indicate the different p21 mRNA expression levels. The results obtained in the real-time detection were also validated by RT-PCR. Analysis of the initial fluorescence increasing rate can efficiently reduce the side effect of enzyme and improve the accuracy in living cell mRNA detection.
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