欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
在最近的年里，蛋白质的特定的察觉是关于在 proteomics 的 aptamers 的热问题之一。这里，我们与双 DNA aptamers 报导了简单、敏感、特定的最近依赖者蛋白质试金。凝血酵素被用作模型蛋白质，和二恰当的 amer 有在第 3'-e 点的互补顺序的探针为蛋白质的二不同 epitopes 被设计。有凝血酵素的二 aptamers 的协会由于第 3'-e 的最近导致了稳定的混血儿，然后，聚合酶反应被导致。获得的 dsDNA 的数量用荧光染料西布尔·格林被显示我。结果证明聚合酶反应的起始的速度与凝血酵素的集中有积极关联。这条 dual-aptamer-based 途径的优点包括了恰当的 amer 探针，高选择和高敏感的简单、灵活的设计。察觉限制是 6.9 pmol/L。
英文摘要：
In recent years, specific detection of proteins is one of the hot issues about aptamers in proteomics. Here we reported a simple, sensitive and specific proximity-dependent protein assay with dual DNA aptamers. Thrombin was used as the model protein, and two aptamer probes with complementary sequence at 3′-end were designed for the two distinct epitopes of the protein. Association of the two aptamers with thrombin resulted in stable hybrids due to the proximity of 3′-end, then polymerase reaction was induced. The amount of obtained dsDNA was indicated using the fluorescence dye Sybr Green I. The results showed that the initial velocity of polymerase reaction had a positive correlation with concentration of thrombin. The advantages of this dual-aptamer-based approach included simple and flexible design of aptamer probes, high selectivity and high sensitivity. The detection limit was 6.9 pmol/L.
同期刊论文项目
同项目期刊论文
Monitoring p21 mRNA expression in living cell based on molecular beacon fluorescence increasing rate
Atomic force microscopy investigation of the characteristic effects of silver ions on Escherichia co
Noninvasive monitoring of intracellular pH change induced by drug stimulation using silica nanoparti
Sensitive fluorescence detection of nucleic acids based on isothermal circular strand-displacement p
Activatable aptamer probe for contrast-enhanced in vivo cancer imaging based on cell membrane
protei
Pyrene-Excimer Probes Based on the Hybridization Chain Reaction
for the Detection of Nucleic Acids i
Electrochemical Detection of Thrombin Based on Aptamer and Ferrocenylhexanethiol Loaded Silica
Nanoc
On-chip oligonucleotide ligation assay using one-dimensional microfluidic beads array for the detect
Telomerase catalyzed fluorescent probes for sensitive protein profiling based on one-dimensional mic
Rhodamine B isothiocyanate doped silica-coated fluorescent nanoparticles (RBITC-DSFNPs)–based biopro
Preparation of luminescent Cy5 doped core-shell SFNPs and its application as a near-infrared fluores
Identification of liver cancer cells in a mixed cell system using galactose-conjugated fluorescent n
Electrochemical detection of nicotinamide adenine dinucleotide based on molecular beacon-like DNA an
Electrochemical biosensors for detection of point mutation based on surface ligation reaction and ol
Barbated Skullcup herb extract-mediated biosynthesis of gold nano-particles andits primary applicati
Fluorescent Nanoparticle-Based Indirect Immunofluorescence Microscopy for Detection of Mycobacterium
A novel molecular beacon based bioassay for highly sensitive and selective detection of NAD+ and the
Chemical etching with tetrafluoroborate: a facile method for resizing of CdTe nanocrystals under mil
期刊信息
位置：