欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
大努力在最近的年里在早诊断和瘤的分子的机制研究上被作了。在这篇论文，基于一个维的微能流动的祷告数组，为瘤的一个平台介绍的联系转移的基因被扔酸开发了在微隧道的功能的祷告。这个平台是敏感的(察觉的限制：0.02 n mol/L ) 并且没有 PCR，能执行 mRNAs 分析。二根人的结肠癌房间线(主要、变形) 从一样，病人被用作在这二根房间正弦瘤介绍联系转移的基因的一个模型，和 transcriptional 表示成功地被完成。而且，在祷告数组上获得的结果被 RT-PCR 验证。这个新奇祷告数组有高敏感，很少样品消费，短试金时间，低费用和高产量能力的优点。它在早诊断和瘤转移的机制研究保持潜力。
英文摘要：
Great efforts have been made on the early diagnosis and molecular mechanism research of tumour metastasis in recent years. In this paper, based on the one-dimensional microfluidic beads array, a novel platform for tumour metastasis-associated genes profiling has been developed by depositing nucleic acids functional beads in the microchannel. This platform is sensitive （limit of detection： 0.02 nmol/L） and can perform mRNAs analysis without PCR. Two human colon cancer cell lines （primary and metastatic） from the same patient were used as a model, and transcriptional expression profiling of multiple tumour metastasis-associated genes in these two cell lines was successfully achieved. Furthermore, the results obtained on the beads array were validated by RT-PCR. This novel beads array has advantages of high sensitivity, little sample consumption, short assay time, low cost and high throughput capability. It holds the potential in early diagnosis and mechanism research of tumour metastasis.
同期刊论文项目
同项目期刊论文
Monitoring p21 mRNA expression in living cell based on molecular beacon fluorescence increasing rate
Atomic force microscopy investigation of the characteristic effects of silver ions on Escherichia co
Noninvasive monitoring of intracellular pH change induced by drug stimulation using silica nanoparti
Sensitive fluorescence detection of nucleic acids based on isothermal circular strand-displacement p
Activatable aptamer probe for contrast-enhanced in vivo cancer imaging based on cell membrane
protei
Pyrene-Excimer Probes Based on the Hybridization Chain Reaction
for the Detection of Nucleic Acids i
Electrochemical Detection of Thrombin Based on Aptamer and Ferrocenylhexanethiol Loaded Silica
Nanoc
On-chip oligonucleotide ligation assay using one-dimensional microfluidic beads array for the detect
Telomerase catalyzed fluorescent probes for sensitive protein profiling based on one-dimensional mic
Rhodamine B isothiocyanate doped silica-coated fluorescent nanoparticles (RBITC-DSFNPs)–based biopro
Preparation of luminescent Cy5 doped core-shell SFNPs and its application as a near-infrared fluores
Identification of liver cancer cells in a mixed cell system using galactose-conjugated fluorescent n
Electrochemical detection of nicotinamide adenine dinucleotide based on molecular beacon-like DNA an
Electrochemical biosensors for detection of point mutation based on surface ligation reaction and ol
Barbated Skullcup herb extract-mediated biosynthesis of gold nano-particles andits primary applicati
Fluorescent Nanoparticle-Based Indirect Immunofluorescence Microscopy for Detection of Mycobacterium
A novel molecular beacon based bioassay for highly sensitive and selective detection of NAD+ and the
Chemical etching with tetrafluoroborate: a facile method for resizing of CdTe nanocrystals under mil
期刊信息
位置：