欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
根据p21基因序列设计合成特异性检测p21mRNA的分子信标,发展了一种体外快速、定量检测p21mRNA的新方法,将其用于肿瘤细胞p21mRNA表达水平的检测.结果发现：抑制p53表达引起CNE2细胞中p21mRNA表达水平降低,转染ING1诱导MCF-7细胞中p21mRNA表达上调,5-氟尿嘧啶处理MCF-7细胞后p21mRNA表达水平呈浓度和时间相关性变化.这种特异性强、操作快速、简便的新方法有望广泛用于各类样品中p21mRNA表达水平检测.
英文摘要：
A molecular beacon,which was designed and synthesized according to the p21 gene sequence,was applied to develop a new method for quantitative detection of p21 mRNA in cellular total RNA.The results showed that p21 mRNA is inhibited by silencing p53 in CNE2 cells while it is upregulated in MCF-7 cells by ING1 transfection,the influence of 5-FU treatment on p21 mRNA of MCF-7 cells is time and concentration-dependent manner.These results demonstrated that the new method with the advantage of high specificity,fast speed and simplicity can be widely applied for p21 mRNA detection in different samples.
同期刊论文项目
同项目期刊论文
Atomic force microscopy investigation of the characteristic effects of silver ions on Escherichia co
Noninvasive monitoring of intracellular pH change induced by drug stimulation using silica nanoparti
Sensitive fluorescence detection of nucleic acids based on isothermal circular strand-displacement p
Activatable aptamer probe for contrast-enhanced in vivo cancer imaging based on cell membrane
protei
Pyrene-Excimer Probes Based on the Hybridization Chain Reaction
for the Detection of Nucleic Acids i
Electrochemical Detection of Thrombin Based on Aptamer and Ferrocenylhexanethiol Loaded Silica
Nanoc
On-chip oligonucleotide ligation assay using one-dimensional microfluidic beads array for the detect
Telomerase catalyzed fluorescent probes for sensitive protein profiling based on one-dimensional mic
Rhodamine B isothiocyanate doped silica-coated fluorescent nanoparticles (RBITC-DSFNPs)–based biopro
Preparation of luminescent Cy5 doped core-shell SFNPs and its application as a near-infrared fluores
Identification of liver cancer cells in a mixed cell system using galactose-conjugated fluorescent n
Electrochemical detection of nicotinamide adenine dinucleotide based on molecular beacon-like DNA an
Electrochemical biosensors for detection of point mutation based on surface ligation reaction and ol
Barbated Skullcup herb extract-mediated biosynthesis of gold nano-particles andits primary applicati
Fluorescent Nanoparticle-Based Indirect Immunofluorescence Microscopy for Detection of Mycobacterium
A novel molecular beacon based bioassay for highly sensitive and selective detection of NAD+ and the
Chemical etching with tetrafluoroborate: a facile method for resizing of CdTe nanocrystals under mil
期刊信息
位置：