欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
以核酸适体作为识别分子，建立了一种简单、灵敏特异的蛋白质检测新方法、该方法以凝血酶为目标蛋白，针对其两个结合位点，分别设计了两条具有3’末端互补碱基序列的核酸适体探针．当两条探针同时与凝血酶结合时，末端借助接近效应形成稳定的杂交并在聚合酶的作用下发生聚合反应，双链DNA产物的量通过Sybr Green Ⅰ的荧光信号指示出来．实验结果表明，聚合反应初速度与凝血酶的浓度正相关．本方法中核酸适体探针设计简单灵活，对目标蛋白选择性和灵敏度高，检测下限可达到6．9pmol／L．
同期刊论文项目
同项目期刊论文
Monitoring p21 mRNA expression in living cell based on molecular beacon fluorescence increasing rate
Atomic force microscopy investigation of the characteristic effects of silver ions on Escherichia co
Noninvasive monitoring of intracellular pH change induced by drug stimulation using silica nanoparti
Sensitive fluorescence detection of nucleic acids based on isothermal circular strand-displacement p
Activatable aptamer probe for contrast-enhanced in vivo cancer imaging based on cell membrane
protei
Pyrene-Excimer Probes Based on the Hybridization Chain Reaction
for the Detection of Nucleic Acids i
Electrochemical Detection of Thrombin Based on Aptamer and Ferrocenylhexanethiol Loaded Silica
Nanoc
On-chip oligonucleotide ligation assay using one-dimensional microfluidic beads array for the detect
Telomerase catalyzed fluorescent probes for sensitive protein profiling based on one-dimensional mic
Rhodamine B isothiocyanate doped silica-coated fluorescent nanoparticles (RBITC-DSFNPs)–based biopro
Preparation of luminescent Cy5 doped core-shell SFNPs and its application as a near-infrared fluores
Identification of liver cancer cells in a mixed cell system using galactose-conjugated fluorescent n
Electrochemical detection of nicotinamide adenine dinucleotide based on molecular beacon-like DNA an
Electrochemical biosensors for detection of point mutation based on surface ligation reaction and ol
Barbated Skullcup herb extract-mediated biosynthesis of gold nano-particles andits primary applicati
Fluorescent Nanoparticle-Based Indirect Immunofluorescence Microscopy for Detection of Mycobacterium
A novel molecular beacon based bioassay for highly sensitive and selective detection of NAD+ and the
Chemical etching with tetrafluoroborate: a facile method for resizing of CdTe nanocrystals under mil
期刊信息
位置：