欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
目的：体外筛选α突触核蛋白可能作用的核苷酸序列。方法根据碱基组成特征合成一系列的核苷酸序列,与α突触核蛋白-绿色荧光蛋白（α-synuclein-green fluorescent protein,α-syn-GFP）融合蛋白混合,采用圆二色谱分析色谱峰的变化情况。结果α突触核蛋白-绿色荧光蛋白融合蛋白混合入GC-box样核苷酸序列后导致色谱峰发生较大变化。结论α突触核蛋白转位进入细胞核后,可能通过作用于GC box样核苷酸序列,参与细胞核内的基因表达调控,从而发挥其生理或病理作用。
英文摘要：
Aim To study the screening of the nucleo-tide sequences might be affected by α-syn in vitro. Methods The nucleotide sequences were synthesized according to the feature of base composition, and then mixed with the α-syn-GFP. The CD was used to ana-lyse the changes of the peak. Result The peak of the CD changed greatly when the α-syn-GFP mixed with the GC-box like sequence. Conclusion The α-syn-GFP might affect the GC-box like sequence after trans-located into the nuclei. Then, it plays a role in physio-logical and pathological conditions by affecting the reg-ulation of gene expression.
同期刊论文项目
同项目期刊论文
Kai-Li Ma, Lian-Kun Song, Yu-He Yuan, Ying Zhang, Ning Han, Kai Gao, Nai-Hong Chen*. The nuclear acc
Yu-he Yuan , Jian-dong Sun, Miao-miao Wu, Jin-feng Hu , Shan-ying Peng, Nai-Hong Chen*. Rotenone Cou
Over-expression of alpha-synuclein 98 triggers intracellular oxidative stress and enhances susceptib
Sun JD, Liu Y, Yuan YH, Li J, Chen NH. Gap junction dysfunction in the prefrontal cortex induces dep
Herpes Simplex Virus 1 ICP22 Inhibits the Transcription of Viral Gene Promoters by Binding to and Bl
The effect of enterovirus 71 immunization on neuropathogenesis and protein expression profiles in th
The Modulation of Phosphatase Expression Impacts the Proliferation Efficiency of HSV-1 in Infected A
期刊信息
位置：