中文摘要：

目的：观察热化疗对淋巴瘤Raji细胞生长的影响及其可能机制。方法：Raji细胞随机分为单纯化疗组（采用3.00mg/L阿霉素处理细胞）、单纯热疗组（采用43℃加热处理细胞）、热化疗组（采用43℃加热联合阿霉素处理细胞）和对照组（细胞不作任何处理）。应用MTT法检测各组细胞增殖率的变化,采用Western Blot检测磷酸化JNK（p-JNK）和热休克蛋白70（HSP70）的表达。结果：对照组、单纯化疗组、单纯热疗组及热化疗组细胞增殖率分别为（100.00±0.00）%、（66.19±2.67）%、（63.44±2.43）%和（58.93±3.66）%,p-JNK的表达量分别为（0.45±0.05）、（0.49±0.04）、（0.48±0.03）和（0.67±0.13）,HSP70的表达量分别为（0.55±0.09）、（1.06±0.84）、（8.23±2.12）和（6.46±2.22）,化疗（a）和热疗（b）及热化疗（a×b）均对以上指标有影响（Fa分别为83.294、17.586和25.176,Fb分别为62.351、14.411和18.553,F（a×b）分别为94.178,25.153和32.189,P均〈0.05）。结论：热化疗联合可抑制Raji细胞增殖,这种抑制作用可能是通过激活JNK信号转导通路或部分抑制HSP70蛋白的表达完成的。

英文摘要：

Aim：To study the effects of thermo-chemotherapy on lymphoma Raji cell growth and its possible mechanism.Methods：Raji cells in chemotherapy group were treated by 3.00 mg/L adriamycin,those in thermotherapy group were treated by heating at 43 ℃ for 30 min,those in thermo-chemotherapy group were treated by 3.00 mg/L adriamycin and heating at 43 ℃ for 30 min,cells without treatment were used as control. MTT assay was used to measure cell proliferation and Western Blot was used to detect the expressions of phosphorylation of JNK（p-JNK） and heat shock protein（HSP）70.Results：The cell proliferation rates of the control group,chemotherapy group,thermotherapy group,and thermo-chemotherapy group were （100.00±0.00）%,（66.19±2.67）%,（63.44±2.43）%,and （58.93±3.66）%,the expression levels of p-JNK of the above groups were （0.45±0.05）,（0.49±0.04）,（0.48±0.03）,and （0.67±0.13）,and the expression levels of HSP70 of the above groups were （0.55±0.09）,（1.06±0.84）,（8.23±2.12）,and （6.46±2.22）. Chemotherapy（a）,thermotherapy（b）,and thermo-chemotherapy（a×b） all had effects on the above parameters（Fa=83.294,17.586,and 25.176;Fb=62.351,14.411,and 18.553;F（a×b）=94.178,25.153,and 32.189,P0.05）.Conclusion：Thermo-chemotherapy can inhibit the growth of lymphoma cells better,and its possible mechanism may be activating JNK signaling pathway or partially inhibiting the expression of HSP70.