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中文摘要：
目的：研究煤焦沥青烟提取物对人肺支气管上皮细胞（BEAS-2B）Nrf2、NQO1 mRNA及Nrf2蛋白表达的影响。方法：煤焦沥青烟提取物以0．00、1．25、2．50、5．00、10．00μg／ml分别染毒BEAS-2B细胞24h后，提取细胞总RNA和总蛋白。采用RT-PCR检测Nrf2、NQO1 mRNA的相对表达量，Western blot测定Nrf2蛋白相对表达量。结果：染毒组BEAS-2B细胞Nrf2 mRNA及蛋白相对表达量均低于对照组，差别均有统计学意义（P均〈0．05），在1．25—5．00μg／ml浓度范围内，随着染毒浓度的增加，Nrf2 mRNA和蛋白表达呈递增趋势，而当染毒浓度为10．00μg／ml时，Nrf2 mRNA和蛋白表达量均有所减少（P〈0．05）。随着染毒浓度的增加，NQO1基因表达水平升高，在5．00、10．00μg／ml时NQO1基因相对表达量比对照组高（P〈0．05）。结论：在煤焦沥青烟提取物浓度较低时（1．25～5．00μg／ml），随染毒浓度增加Nrf2表达水平升高，可能上调NQO1基因的表达，增强BEAS-2B细胞氧化应激能力。
英文摘要：
OBJECTIVE： To study the expressions of Nrf2 and NQO1 mRNA, Nrf2 protein by coal tar pitch （CTP） extract. METnOOS： BEAS-2B cells were treated with different concentrations of CTP extract （0.00, 1.25, 2.50, 5.00, 10.00 μg/ml） for 24 h. Total cell RNA and protein were extracted. The expressions of Nrf2 and NQO1 mRNA and Nrf2 protein were measured by the reverse transcription polymerase chain reaction （RT-PCR） and Western blot, respectively. RESULTS： The expressions of mRNA and protein of Nrf2 among test groups（1.25- 5.00 μg/ml） were significantly lower than those in the control group. With increasing concentration of CTP extract, the expressions of Nrf2 and NQO1 mRNA, Nrf2 protein increased. However, the mRNA and protein of Nrf2 decreased in the CTP 10.00 μg/ ml group. CONCLUSION： When the concentration of CTP extract was at a low level,the expression of Nff2 increased in parallel with the CTP extract concentration. So Nrf2 might induce the gene expression of NQO1, enhancing the antioxidant ability of BEAS-2B cells.
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