中文摘要：

目的 探讨DNA修复基因XRCC1多态对肺癌发生易感性的影响.方法 采用病例对照研究的方法选择209例肺癌患者为病例组,256例健康检查者为对照组,应用聚合酶链反应-限制性片段长度多态性（PCR-RFLP）技术检测XRCC1基因194、280和399位点的多态性.结果 病例组与对照组XRCC 1-194位点Trp/Trp变异基因型分布频率分别为19.1％、10.9％,两组比较,差异有统计学意义（P＜0.05）.该变异基因型携带者发生肺癌的危险度是野生型携带者的2.215倍（95％CI为1.276～3.845）.病例组与对照组XRCC1-280位点Hi,His变异基因型分布频率分别为6.7％、4.3％,两组比较,差异有统计学意义（P＜0.05）.该变异基因型携带者发生肺癌的危险度是野生型携带者的2.460倍（95％CI为1.141～5.304）.病例组与对照组XRCC 1-399位点Gln/Gln基因型分布频率分别为10.0％、9.0％,两组比较,差异无统计学意义（P＞0.05）.XRCC1-194、XRCC 1-280多态基因位点与吸烟在肺癌发生中均存在交互作用,携带XRCC1-194 Arg/Trp＋Trp＋Trp基因型和XRCC 1-280 His/His＋Arg/His基因型的吸烟人群患肺癌的危险度分别为4.889（95％CI为2.828～8.452）和6.281（95％CI为3.572～11.046）,明显高于携带野生基因型的非吸烟者.结论 携带XRCC1-194 Trp/Trp和XRCC 1-280 His/His突变等位基因及其与吸烟的交互作用使肺癌发生的风险增加,XRCC1基因多态与吸烟在肺癌的发生过程中起一定作用

英文摘要：

Objective To explore the relationship between the polymorphisms of DNA repair gene XRCC1 and susceptibility to pulmonary cancer.Methods A case-control study of 209 lung cancer patients and 256 control subjects was conducted to investigate the role of XRCC1 gene in lung cancer.Genotyping was performed using PCR based restriction fragment length polymorhphism（PCR-RFLP）technique.Results The frequency（19.1%）of XRCC 1-194 Trp/Trp in case group was significantly higher than that（10.9%）in control group（P〈0.05）,OR for lung cancer was 2.215（95%CI： 1.276-3.845）.The frequency（6.7%）of XRCC1-280His/His in case group was significantly higher than that（4.3%）in control group（P〈0.05）,OR for lung cancer was 2.46（95%CI： 1.141 ～5.304）.There was no significant difference for XRCC1-399 Gln/Gln genotype between the two groups.Interaction analysis of gene polymorphisms and environment factors indicated that there was interactions between XRCC1-194 Trp/Trp and XRCC1-280 His/His genotypes and smoking.The risks of lung cancer in smokers with XRCC 1-194 Arg/Trp＋Trp/Trp and XRCC 1-280 His/His＋Arg/His were 4.889（95%CI： 2.828～8.452）and 6.281（95%CI：3.572～11.046）,respectively.Conclusion These findings supported the hypothesis that the interaction of polymorphisms of XRCC1-194 Trp/Trp,XRCC1-280 His/His with smoking resulted in the increased risk of lung cancer,and the polymorphisms of XRCC and smoking could play an role in development of lung cancer.