中文摘要：

目的 观察非小细胞肺癌患者血清中组蛋白去乙酰化酶1（HDAC1）和DNA甲基转移酶1（DNMT1）蛋白的表达,探讨其临床意义.方法 采用酶联免疫吸附试验（ELISA）法对2012年9月至2013年6月郑州大学第一附属医院呼吸内科136例原发性肺癌患者（肺癌组）和同期郑州市第六人民医院进行健康体检的147名健康人群（对照组）血清中HDAC1和DNMT1蛋白浓度进行测定,分析其与临床病理特征的关系.结果 肺癌组血清中HDAC1和DNMT1蛋白浓度分别为（145±53）ng/L和（50±11）μg/L,显著高于对照组的（78±56）ng/L和（27±6）μg/L（t=596.16、152.64,均P=0.000）;HDAC1和DNMT1蛋白浓度与性别、年龄、吸烟史均有显著相关性（均P＜0.05）;HDAC1与DNMT1蛋白浓度呈正相关（r=0.525,P=0.000）;不同组织学类型和不同临床分期肺癌患者血清HDAC1和DNMT1蛋白浓度差异均有统计学意义（均P＜0.01）.结论 血清中HDAC1和DNMT1蛋白高表达可能增加患非小细胞肺癌的危险性,可能在肺癌发展早期过程中起重要作用.

英文摘要：

Objective To detect the serum protein expressions of histone deacetylase 1 （ HDAC1 ） and DNA methyltransferase 1 （DNMT1） in patients with non-small-cell lung cancer and explore the correlation between their expressions and clinicopathologieal features. Methods Enzyme-linked immunosorbent assay （ELISA） was used to measure the protein expressions of HDAC1 and DNMT1 in 136 lung cancer patients hospitalized at Department of Respiratory Diseases, First Affiliated Hospital, Zhengzhou University from September 2012 to June 2013 （cancer group）. And 147 healthy physical examination subjects from Sixth People＇s Hospital of Zhengzhou were selected as controls and their clinicopathological features analyzed. Results The protein expressions of HDAC1 and DNMT1 in patients with lung cancer （145 ~ 53,50 ~ 11 ） were higher than those in control group （78 ＋ 56,27 ＋ 6 ）. And there was statistical significance（ t = 596. 16,152. 64, both P = 0. 000 ）. The protein expressions of HDAC1 and DNMT1 were correlated with gender, smoking history and age （ among P 〈 0. 05 ）. There was a positive association between the protein expressions of HDAC1 and DNMT1 in non-small-cell lung cancer （ r = 0. 525, P = 0. 000 ）. Statistical significance existed between the protein expressions of HDAC1 and DNMT1 and histological types or different stages （ all P 〈 0.01 ）. Conclusion The higher protein expressions of HDAC1 and DNMT1 may play an important role in the early process of lung cancer.