中文摘要：

从四川省各市县收集猪肉样品130份,选择性培养基分离大肠杆菌后VITEK进行鉴定,采用K-B法药敏实验测试大肠杆菌对10种药敏纸片的耐药性.琼脂稀释法测定大肠杆菌对3种季铵盐消毒剂的MIC值,PCR扩增10种季铵盐类消毒剂耐药基因.结果表明：130份猪肉样品中分离大肠杆菌96株,分离率为73.85％,其中73株对抗生素产生耐药性,耐药率分别为：TET （64.58％）、AMP （37.50％）、S（32.29％）、K（21.88％）、KF （20.83％）、CIP （18.75％）、CN （9.38％）、SAM （6.25％）、CAZ （2.08％）、CRO （2.08％）,共产生了28种耐药谱,TET是最主要的谱型;96株大肠杆菌对季铵盐消毒剂BC、DDAC、CTAB的MIC分别为：16～64 μg/mL、8～32 μg/mL、64～256 μg/mL; QACs耐药基因检出率分别为ydgE/F （81.25％）、mdfA （50％）、sugE （c） （45.83％）、emrE （36.46％）、qacE△1 （19.79％）、qacF （17.71％）、qacE （14.58％）、sugE（p）（3.13％）,qacG-未检出.共检出42种消毒剂耐药基因组合（1.04％～12.50％）.sugE （c）、qacF基因与氨基糖苷类及AMP耐药相关,qacE△1基因与AMP耐药相关.四川省肉源大肠杆菌污染情况较严重,菌株对抗生素的耐药率及多重耐药相对较低,对季铵盐类消毒剂MIC较高,消毒剂耐药基因检出率较高,应引起足够重视,加强对其检测.

英文摘要：

Escherichia coli strains were isolated from 130 pork samples collected in Sichuan province using selective medium and confirmed by VITEK.The susceptibility of the isolated E.coli strains to ten antibiotics was tested according to the standard disk diffusion method of the Clinical and Laboratory Standards Institute （CLSI）.The minimum inhibitory concentrations （MICs） of three quaternary ammonium compounds （QACs） against these strains were determined by an agar dilution method.QAC resistance genes were amplified using ten different sets of primers.The results showed that 96 E.coli strains were obtained with frequency of 73.85％.The resistance frequency to tetracycline （TET）,ampicillin （AMP）,streptomycin （S）,kanamycin （K）,ceftofur （KF）,ciprofloxacin （CIP）,gentamiein （CN） ampieillin/sulbactam （SAM）,ceftazidime （CAZ） and ceftriaxone （CRO） was 64.58％,37.50％,32.29％,21.88％,20.83％,18.75％,9.38％,6.25％,2.08％ and 2.08％,respectively.There were 28 different antibiotic resistance profiles Among these,TET profile was the dominant one.The MICs of benzalkonium chloride （BC）,didecyldimethylammonium chloride （DDAC） and etrimonium bromide （CTAB） against E.coli were 16-64 μg/mL,8-32 μg/mL,and 64-256 μg/mL,respectively.ydgE/F （81.25％） was the most prevalent QAC resistance gene in E.coli,followed by mdfA （50％）,sugE（c） （45.83％）,emrE （36.46％）,qacE△1 （19.79％）,qacF （17.71％）,qacE （14.58％） and sugE（p） （3.13％）.And qacG-was not detected in any of the isolates.There were 42 different qac resistance gene groups （1.04％-12.50％）.Both sugE（c）and qacF gene were commonly present in aminoglycoside and AMP resistant E.coli,and qacE△1 gene was also related with AMP resistance.The resistance frequency and multi-drug resistance were lower than those of other reports.The frequency of QAC resistance genes and the MIC of QACs were high,suggesting that the E.coli from pork may be a reservoir of antibacteri