中文摘要：

为了解猪萨佩罗病毒（porcine sapelovirus,PSV）的流行及变异情况,采集2015-2016年四川地区12个县市34个猪场共计428份腹泻病料,采用QRT-PCR方法对PSV进行检测。结果显示：在428份病料中,共有114份为PSV阳性,阳性率为26.6%;所检测的34个猪场中,共有20个猪场显示PSV阳性,猪场阳性率为58.8%,表明PSV在四川地区普遍存在。此外,对来自不同县市共14份PSV阳性病料1B全基因进行PCR扩增,通过分子克隆,测序,再利用序列分析软件对四川部分地区猪萨佩罗病毒1B基因进行序列分析。结果表明,14条猪萨佩罗病毒1B基因核苷酸序列及推导出的氨基酸序列相似性分别为90.6%~100%和97.1%~100%。遗传进化分析显示,该研究获得的四川株与已知中国株分属于一个大的分支,说明我国猪萨佩罗病毒1B基因序列较为保守,没有发生大的基因变异。所有中国分离株与韩国株、德国株、英国株处在不同分支,又说明我国PSV的流行毒株可能存在独立进化。

英文摘要：

To investigate the prevalence of porcine sapelovirus（PSV）,a total of 428 fecal samples from pigs with diarrhea were collected from 34 pig farms during 2015 to 2016 in Sichuan Province,and PSV in these samples was detected by using qRT-PCR. The results revealed that 114（26. 6%） samples and 20（58. 8%） farms were positive for PSV,which indicated that PSV infections in pig herds were endemic in Sichuan province. The 14 novel PSV 1B nucleotide sequences were obtained by RT-PCR and confirmed by sequencing（Gen Bank accession No. ： KU323644-KU323657）. Nucleotide and the deduced amino acid sequence homologies were 90. 6%-100% and 97. 1%-100%,respectively. Phylogenetic analysis indicated that the novel Sichuan strains belong to a group with the strains from other provinces of China. There was no major gene mutation,which showed that the gene of PSV was highlyconserved in China. The novel Sichuan strains,Korea strains,Germany strains and Britain strains were in different branches,which could indicate that the prevalence of PSV in our country might be independent evolution.