中文摘要：

为建立快速检测猪环曲病毒（PToV）的RT-LAMP方法,根据GenBank中发表的N蛋白基因序列,设计合成4条特异性LAMP引物,通过敏感性试验和特异性试验,建立了PToV RT-LAMP检测方法,并对71份临床粪便进行了检测应用。结果显示,本试验建立的PToV RT-LAMP检测方法在65℃60min条件下可扩增出特异性梯状DNA条带,与猪圆环病毒2型、猪瘟病毒、伪狂犬病病毒、猪繁殖与呼吸综合征病毒、猪传染性胃肠炎病毒、猪传染性胃肠炎病毒、猪细小病毒、猪环曲病毒的核酸无交叉反应;检测极限为10copies/μL,比普通RT-PCR的灵敏度高10倍,表明该检测方法具有较强的特异性及灵敏性。用建立的RT-LAMP方法进行的样品检测结果显示,受检的71份样品中有17份检测结果为阳性,该结果与常规RTPCR检测结果吻合,总的阳性率为23.9%。

英文摘要：

To develop a reverse transcription loop-mediated isothermal amplification（RT-LAMP）method for quick detection of porcine torovirus（PToV）,4specific RT-LAMP primers were designed and synthesized according to the conserved sequences of N protein gene of PToV published in GenBank,meanwhile its specificity and sensitivity were assessed,and 71 clinical samples were detected using the RTLAMP and RT-PCR in paralle.In result,a ladder like bands of PToV were amplified using the RT-LAMP method,performed at 65 ℃for 60 min.The detection limitation reached 10copies/μL,which was 10-fold higher than that of the traditional RT-PCR;and there was no cross-reaction with PCV2,CSFV,PRV,PRRSV,PEDV,TGEV,PPV and PKoV.PToV positive rate in the clinical samples was 23.9%（17/71）.These results indicated a good correlation between PToV RT-LAMP and RT-PCR.