中文摘要：

磷脂二酰甘油酰基转移酶（phospholipids：diacylglycerol acyltransferase,PDAT1）是植物三酰甘油（triacylglycerol,TAG）合成的关键酶。本文在甘蓝型油菜湘油15号c DNA中克隆到3个PDAT1全长编码序列（coding sequence,CDS）,经比对分别定位于A02、A10、C09染色体,分别命名为Bn PDAT1-A02、Bn PDAT1-A10和Bn PDAT1-C09,其序列长分别为1998、2002和2005 bp,各自编码665、666、667个氨基酸。预测Bn PDAT1基因编码蛋白定位于细胞质膜,具有典型的PDAT1保守结构域。多序列比对和进化分析表明,Bn PDAT1基因编码蛋白与甘蓝、拟南芥、亚麻芥PDAT1蛋白具有较高的同源性。酵母互补实验证实,该基因编码蛋白具有PDAT1酶活性。Bn PDAT1基因在湘油15号中的表达现先上升后降低趋势,在开花后25~30 d达最大值,但3个拷贝的表达变化规律存在差异。

英文摘要：

Phospholipids：diacylglycerol acyltransferase（PDAT1） is a key enzyme in triacylglycerol（TAG） biosynthesis of plants. In this study, three novel PDAT1 coding sequences（CDSs） were isolated from c DNA of Brassica napus L. cv. Xiangyou 15 seeds, which were mapped to the chromosomes A02, A10, and C09, and designated as Bn PDAT1-A02, Bn PDAT1-A10, and Bn PDAT1-C09, respectively. Three Bn PDAT1 CDSs were 1998, 2002, and 2005 bp in length and encoded predicted proteins with 665, 666, and 667 amino acid residues, respectively. Bn PDAT1 proteins were predicted to be located on the cell membrane and have a typical PDAT1 conserved domain. Multiple sequence alignments and phylogenetic analysis showed that the deduced amino acid sequences of Bn PDAT1 were highly homologous to previously reported PDAT1 in Brassica oleracea, Arabidopsis thalian, and Eruca sativa. Furthermore, the catalytic enzyme activity of the cloned Bn PDAT1 genes was confirmed by the yeast complementary experiment. The expression level of Bn PDAT1 s increased gradually in seed development and reached the maximum from 25 to 30 days after flowering. However, three Bn PDAT1 copies were also found to be different in expression pattern.