中文摘要：

目的建立紫金龙药材中8-deacetyl-yunaconitine的含量测定方法,为其质量标准的建立提供科学依据。方法于2015年1—6月,选择贵州不同产地紫金龙药材21批,采用超高液相色谱（UPLC）的方法,色谱柱Agilent Eclipse XDB-C18柱,流动相为乙腈-0.1%醋酸铵,梯度洗脱,流速1.0 m L/min,检测波长254 nm。记录8-deacetyl-yunaconitine的色谱峰面积。结果测定了21批不同产地的紫金龙药材,8-deacetyl-yunaconitine含量在0.054%~0.250%。平均回收率为99.6%,RSD为0.6%。结论该方法简便、快速、准确、重复性好,可以控制该药材的质量。

英文摘要：

Objective To develop an UPLC method for determination of 8-deacetyl-yunaconitine in Aconitum vilmorinianum Kom. Methods 21 samples of Aconitum vilmorinianum Kom from January to June 2015 in diffrent palces were selected. Analysis was carried out on a Agilent Eclipse XDB-C18 analytical column, and eluted with a gradient solvent system of acetonitrile-0.1% ammonium acetate as the mobile phaser. The detection wave length was set at 254 nm. The chromatographic peak area of 8-deacetyl-yunaconitine was recorded. Results The contents of in 21 samples of Aconitum vilmorinianum Kom were in the ranges of 0.054%~0.250%. The average recovery rate was 99.6%, RSD Was 0.6%. Conclusion The method is simple, accurate and reproducible, and is suitable for the quality control of Aconitum vilmorinianum Kom.