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中文摘要：
目的：对粘质沙雷氏菌（semtiamarcescens）XJ-01几丁质酶合成条件优化。方法：研究了碳源、氮源、温度、pH等单个因素对该菌几丁质酶合成的影响，并通过正交实验确定了该菌的最适酶合成条件。结果：该菌几丁质酶最优合成条件为：胶体几丁质5g／L、硫酸铵5gL、培养温度32℃、最适pH8。结论：优化了S.marcescens XJ-01几丁质酶的合成条件。
英文摘要：
Objective： To determine the optimal conditions for chitinase production by Serratia marcescens XJ-01. Methods： The effects of culture conditions （e.g. carbon source, nitrogen source, temperature and pH） for chitinase production by Serratia marcescens XJ-01 were investigated by orthogonal experiment. Results： show that the optimal condition for producing chitinase is as follows： temper-ature of 32℃, pH 8.0, 5 g/L （NH4）2SO4 as the nitrogen source and 5 g/L colloidal chitin as the carbon source. Conclusions： The optimal conditions for chitinase production by Serratia marcescens were determined.
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