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Bioleaching of low-grade copper sulphides

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分类：TQ245.24[化学工程—有机化工] X171.5[环境科学与工程—环境科学]
作者机构：[1]School of Minerals Processing and Bioengineering, Central South University, Changsha 410083, China, [2]State Key Laboratory of Pollution Control and Resources Reuse, Nanjing University, Nanjing 210008, China, [3]School of Life Science and Technology, Huazhong University of Science and Technology, Wuhan 430074, China
相关基金：Foundation item： Project（50621063） supported by the National Nature Science Foundation of China; Project（NCET-06-0691） supported by the Program for New Century Excellent Talents in University
相关项目：应力波作用下溶浸液在散体介质中的流动机理研究

关键词：邻苯二甲酸二丁酯, 好氧生物降解, 沉积物, 微生物分析, 湘江, 限制性片段长度多态性, 高效液相色谱法, 生物降解率, bioremediation, di-n-butyl phthalate, sediment, polymerase chain reaction-restriction fragment length polymorphism, microbial diversity

中文摘要：

Di-n-butyl phthalate (DBP ) ， phthalate 酸酉旨(PAE ) 之一，被调查用 Xiangjiang 河沉积决定它的简历降级率并且在沉积的丰富文化发现潜在的 DBP degraders。沉积样品为 5 d 与 100 mg/L 的 DBP 的起始的集中被孵化。DBP 的简历降级率用 HPLC 被检测，降级的产品被 GC/MS 分析。随后，丰富文化的微生物引起的差异被聚合酶链反应限制碎片长度多型性(PCR-RFLP ) 分析。结果表明 DBP 的几乎 100% 被降级在以后仅仅 3 d，产生二个主要降级的产品：单音丁基的 phthalate (MBP ) 和 9-octadecenoic 酸。在在 DBP 的压力下面的一个六月的丰富时期以后，在最后的丰富文化的主导的家庭与 Comamonas sp 被聚类，留下隶属于 Sphingomonas sp， Hydrogenophaga sp，根瘤细菌属 sp，和 Acidovorax sp。结果显示出在环境在 DBP 的简历补习要过去常的这些细菌的潜力。

英文摘要：

Di-n-butyl phthalate （DBP）, one of phthalate acid esters （PAEs）, was investigated to determine its biodegradation rate using Xiangjiang River sediment and find potential DBP degraders in the enrichment culture of the sediment. The sediment sample was incubated with an initial concentration of DBP of 100 mg/L for 5 d. The biodegradation rate of DBP was detected using HPLC and the degraded products were analyzed by GC/MS. Subsequently, the microbial diversity of the enrichment culture was analyzed by polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP）. The results reveal that almost 100% of DBP is degraded after merely 3 d, generating two main degraded products： mono-butyl phthalate （MBP） and 9-octadecenoic acid. After a six-month enrichment period under the pressure of DBP, the dominant family in the final enrichment culture is clustered with the Comamonas sp., the remaining are affiliated with Sphingomonas sp., Hydrogenophaga sp., Rhizobium sp., and Acidovorax sp. The results show the potential of these bacteria to be used in the bioremediation of DBP in the environment.