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中文摘要：
目的：测定不同产地、不同部位千层塔药材中石杉碱甲和石杉碱乙的含量。方法：采用UItimate XB-C18（4.6 mm×250 mm,5μm）色谱柱,流动相为乙腈-甲醇-水（10∶55∶240）,含0.32%磷酸和0.5%三乙胺,流速1.0 mL.min-1,检测波长309 nm,柱温25℃。结果：测得各地千层塔中石杉碱甲、石杉碱乙最高含量分别为0.0900%和0.0183%,各地石杉碱甲、石杉碱乙最低含量分别为0.0014%和0。结论：所测定的方法简便、可靠、快速,重复性好,结果稳定,适用于千层塔药材中石杉碱甲和石杉碱乙的同时快速含量测定,该研究可为千层塔最佳产地及最佳药用部位的确定提供参考。
英文摘要：
Objective： To develop an HPLC method for simultaneous determination of huperzine A （ Hup - A） and huperzine B （Hup -B） in different parts of Huperzia serrate from different habitats. Methods：The assay was per- formed on an Uhimate XB -C18（4.6 mm ×250mm,5 μm） column with the mobile phase of aeetonitrile- metha- nol - water （ 10 ： 55 ： 240 ） containing 0. 32 % phosphoric acid and 0.5 % triethylamine. The flow rate was 1.0 mL .min-1 ,the detection wavelength was set at 309 nm, and the column temperature was set at 25 ℃. Re- sults：The contents of huperzine A and huperzine B in Huperzia serrata collected at different habitats were evidently different. The maximum contents of huperzine A and huperzine B were 0. 0900% and 0. 0183% , respectively;the minimum contents of huperzine A and huperzine B were 0. 0014% and 0,respectively. Conclusions：The method is simple, accurate and repeatable, which provides a reliable way for evaluating the quality of Huperzia serrata by meas- uring huperzine A and huperzine B, as well as provide a reference for the determination of the best origin and the most rational part of the plant.
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