分别采用乙醇、乙酸乙酯、甲醇3种有机溶剂提取发酵液中的麦考酚酸(MPA),分析紫外光谱吸收特征,并建立了测定MPA含量的紫外分光光度法.结果表明:MPA甲醇溶液质量浓度在0-50mg/L范围内与Ab304呈良好的线性关系,平均回收率为101.8%,相对标准偏差为1.20%.紫外分光光度法测定MPA含量简单、快速、重复性好,适用于发酵液中MPA含量的准确测定.
A UV speetrophotometrie method to determinine mycophenolic acid in zymofic fluid using ethanol, ethylaeetate and methanol as extraction was established. The result showed: the linearity was in the range of 0 -50 mg/L of MPA methanol solution, the average recovery was 101.8 %, and RSD was 1.20 %. The method was simple, rapid and reproducible, and it was suitable for the determination of mycophenolic acid in fermentation broth.