欢迎您！东篱公司
退出
-
申报数据库
-
立项数据库
-
成果数据库
-
项目获奖数据库
中文摘要：
目的：观察兔视神经损伤后视网膜神经节细胞（retinal ganglion cell，RGC）丙二醛（malondialdehyde，MDA）／超氧化物歧化酶（superoxide dismutase，SOD）的变化。方法：选用新西兰白兔32只，分为对照组和损伤组，损伤组再根据损伤后不同时间分为3，7，14d组，每组8只16眼。采用硫代戊巴比妥酸法和黄嘌呤氧化法分别测定视网膜MDA／SOD含量。结果：损伤实验组MDA含量分别为5．95±0．78，7．67±0．64和8．29±1．02μmol／g，均明显高于正常对照组（3．82±0．54μmol／g）；实验各组SOD含量分别为510±44，415±29和398±36μkat／g，均明显低于正常对照组（727±52μkat／g）。结论：自由基代谢在兔视神经损伤后RGC凋亡中起着重要作用，MDA／SOD含量测定在RGC凋亡的研究中有一定的意义。
英文摘要：
AIM： To study the changes of MDA/SOD in retinal ganglion cell（RGC） after optic nerve injuries in rabbits. METHODS： Thirty-two New Zealand rabbits without eye diseases were divided into two groups randomly： normal control group（ n = 8, 16 eyes） ; injury group（ n = 24, 48 eyes）. The injury group was divided into three sub groups according to different time points： 3-day group, 7-day group and 14-day group （n = 8, 16 eyes, respectively）. MDA/SOD was measured respectively by TBA and xanthine oxidase. RESULTS： The content of MDA in injury groups were 5.95 ± 0. 78, 7. 67 ± 0. 64 and 8. 29 ± 1. 02μmol/g respectively, which was higher than the normal control group（3.82 ± 0.54μ mol/g）. The content of SOD in injury groups were 510 ± 44, 415 ± 29 and 398 ± 36μkat/g respectively, which was lower than the normal control group （727 ± 52μ kat/g）. CONCLUSION： Free radical metabolism plays an important role in RGC apoptosis after optic nerve injuries in rabbits and the determination of MDA/SOD had certain application value in the study of RGC apoptosis.
同期刊论文项目
同项目期刊论文
期刊信息
位置：