中文摘要：

将单核细胞株THP-1按照随机数字表随机分为观察组和对照组两纽．正常的观察组给予肉苁蓉多糖培养液，根据肉苁蓉多糖浓度不同又分为10、50、100μg/L组，对照组不添加肉苁蓉多糖培养液，实验组中的不同浓度组、对照组，每组各24例．比较各组THP-1细胞吞噬率、吞噬指数以及细胞因子分泌水平．以此研究肉苁蓉多糖对THP-1细胞吞噬作用的影响并探讨其发生机理．结果显示，观察组中10、50、100μg／L组单核巨噬细胞吞噬率分别为（17．42±3．19）％、（23．32±3．82）％、（29．41±4．11）％，均高于对照组的（9．34±1．26）％，比较差异有统计学意义（F=162．63，P〈0．01）；10、50、100μg／L组单核巨噬细胞吞噬指数分别为（0．45±0．13）、（0．67±0．19）、（0．82±0．21），均高于对照组的（0．22±0．09），比较差异有统计学意义（F=62．60，P〈0．01）；10、50、100μg／L组IFN-γ分别为（4．21±1．11）、（7．38±1．86）、（9．51±2．10）mg／L，均高于对照组（0．79±0．06）mg／L，比较差异有统计学意义（F=152．74，P〈0．01）；10、50、100μg/L组IL-1分别为（24．11±4．15）、（74．60±11．21）、（103．61±19．65）mg/L，均高于对照组（10．21±1．39）mg／L，比较差异有统计学意义（F=343．15，P〈0．01），各指标进一步采用Newman—Keals法进行两两比较均有统计学意义（P〈0．01）．由此认为，肉苁蓉多糖可能通过增强THP-1细胞吞噬能力和促细胞因子释放发挥免疫调节功能．

英文摘要：

The monocytic cell line THP-1 were randomly divided into observation group and control group. According to the different Cistanche desertico/a polysaccharide （CDPS） concentration, the observation groups were divided into 10, 50, 100 p,g/L groups; Control group without the addition of CDPS medium. 24 cases in the observation group and 24 cases in the control group. Comparison of THP-1 cell phagocytosis rate, phagocytic index and cytokine secretion levels to explore its mechanism and effects of Cistanche phagocytosis polysaccharides on THP-1 cells. The results showed： the phagocytic rate of 10, 50, 100 μg/L groups of mononuclear macrophage phagocytic rate were （17.42±3.19）%, （23.32±3.82）%, （29.41±4.11）%, higher than that of the control group （9.34±1.26）% with a statistically significant difference （F=162.63, P〈0.01）; The phagoeytosis indexes of 10, 50, 100 μg/L groups of mononuclear macrophage were（0.45±0.13）, （0.67±0.19）, （0.82± 0.21）, also higher than that of the control group （0.22±0.09） with a statistically significant difference （F=62.60, P〈 0.01）; IFN-T of 10, 50, 100 μg/L groups were（4.21_±1.11）, （7.38±1.86）, （9.51±2.10） mg/L, being higher than those in the control group （0.79_±0.06） mg/L with a statistically significant difference（F=152.74, P〈0.01）; Moreover, the value of IL-1 of 10, 50, 100 Ixg/L groups were （24.11±4.15）, （74.60±11.21）, （103.61±19.65） mg/k which were also higher than those in the control group （10.21±1.39） mg/L with a statistically significant difference（F=343.15, P〈0.01）. The difference of each index calculated further by using Newman-Keals method were again sta-tistieally significant difference（P〈0.01）. Therefore, it is concluded that Cistanche polysaeeharide may enhance the phagocytic ability of THP-1 eells and promote the release of cytokines in immune regulation function.