目的:研究海绵Phakellia fusca共附生真菌的次级代谢产物。方法:采用正相硅胶柱色谱、SephadexLH-20凝胶柱色谱、制备薄层、重结晶等方法进行分离和纯化,并根据其理化性质及波谱数据鉴定结构。结果:分离得到4个化合物,分别鉴定为:环(L-缬-L-脯)二肽(1)、环(L-苯丙-L-脯)二肽(2)、环(L-酪-L-脯)二肽(3)、环(24-羟基-15-甲基癸酰-甘-缬-亮-丙-苯丙)(4)。结论:化合物1~4为环肽类化合物,均为首次从该真菌中分离得到。
Objective: To study the secondary metabolites from the marine sponge Phakellia fusca epiphytic fungi.Methods: The compounds were isolated by column chromatography over silica gel and purified by Sephadex LH-20 column chromatography and preparative TLC.The structures were elucidated by means of physiochemical properties and spectroscopic analyses.Results: Four compounds were separated and identified as: cyclo-(L-Val-L-Pro)(1),cyclo-(L-Phe-L-Pro)(2),cyclo-(L-Tyr-L-Pro)(3),cyclo-(3-hydroxy-4-methyldecanoyl-Gly-L-Val-D-Leu-L-Ala-L-Phe)(4).Conclusion: Compounds 1~4 are obtained from the marine sponge Phakellia fusca epiphytic fungi for the first time.