采用高速逆流色谱从花椒萃取物中纯化花椒麻味素标准品。模拟厨房水煮、油煎条件,高效液相色谱外标法定量分析,研究了水煮、热油处理对花椒麻味素含量的影响。结果表明,热油处理时花椒麻味素的浸出量远高于水煮,但其热损失也大;随着水煮或热油处理的时间增长,萃取出的花椒麻味素量均呈现先增加后减小的趋势;油温升高,花椒麻味素损失显著增大,油温控制在〈190℃较好。传统烹饪中,水煮1.5h较好,煮出的麻味物质含量高;热油处理时油温150-180℃时间0.5h较好,油液中麻味物质含量高。
The tingling - taste components of hydroxyl - sanshools were purified from the extracting of paprika Zanthoxy- lure Bungeanum Maxim. by high - speed counter - current chromatography. To simulate the traditional Chinese cooking process, the Zanthoxylum Bungeanum Maxim. was added in boiling water for different hours or hot oil in different tem- peratures and different hours. The tingling - taste components changes in paprika and in the liquid of water or oil were quantified by HPLC using the pure tingling - taste components as the external standard. The conclusions were : ( 1 ) the tingling - taste components extracted more by hot oil than by boiling water. ( 2 ) With the increase of processing hours in boiling water or in hot oil, the content of tingling - taste components extracted in the liquid of water or oil was first up then down. ( 3 ) With the increase of temperature of hot oil, the amount of tingling - taste components extrac- ted in the oil was significantly reduced, it' s better to control the hot oil temperature below 190℃. (4) It seemed that for the traditional Chinese cooking practice, handling 1.5h by boiling -water or 150 - 180℃ and 0. 5h by hot oil was optimal to extract the tingling - taste substances in the pericarps of Zanthoxvlum Bungeanum Maxim.