中文摘要：

目的研究氨基胍（AG）对乳酸盐腹膜透析液（L-PDS）致人腹膜间皮细胞（HMr SV5）损伤及血管内皮生长因子（VEGF）表达的影响。方法采用人腹膜间皮细胞株HMr SV5为体外实验模型,分为无血清DMEM培养液对照组、L-PDS组（2.5%L-PDS）、L-PDS＋AG组（2.5%L-PDS＋10 mmol/L AG）、单纯AG组（终浓度10 mmol/L）。各组以上不同干预因素与HMr SV5细胞共孵育。MTT法检测各组细胞增殖、评估细胞活力,Western blot和逆转录-聚合酶链反应（RT-PCR）检测VEGF蛋白及m RNA的表达。结果 MTT试验表明,L-PDS组OD值为（0.120±0.019）,明显低于对照组的（0.298±0.031）,差异有统计学意义（P〈0.05）;L-PDS＋AG组OD值为（0.289±0.022）,明显高于LPDS组,差异有统计学意义（P〈0.05）。Western blot和RT-PCR结果均表明,与对照组比较,L-PDS组细胞中VEGF蛋白和m RNA表达明显增加,与L-PDS组比较,PDS＋AG组VEGF蛋白和m RNA的表达明显减低,差异均有统计学意义（P〈0.05）。结论 AG可拮抗乳酸盐腹膜透析液致人腹膜间皮细胞损伤并下调VEGF的表达。

英文摘要：

Objective To investigate the effect of aminoguanidine（AG） on damage in human peritoneal mesothelial cells（HMr SV5） caused by lactate peritoneal dialysis solution（L-PDS） and expression of vascular endothelial growth factor（VEGF）. Methods Depending on the culture of the cell, the cultured HMr SV5 cells were divided to control group（cultured in DMEM）, L-PDS group（2.5% L-PDS）, L-PDS＋AG group（2.5% L-PDS＋10 mmol/L AG）, and AG group（at a final concentration of 10 mmol/L）. MTT assay was used to assess the viability and proliferation of the cells.VEGF protein was determined by western blot, and VEGF m RNA was determined by RT-PCR. Results The optical density（OD） value in L-PDS group was（0.120±0.019）, significantly lower than（0.298±0.031） in the control group（P〈0.05）. The OD value in L-PDS＋AG group was（0.289±0.022）, which was significantly higher than that in L-PDS group（P〈0.05）. Expression of VEGF protein and m RNA increased significantly in L-PDS group as compared with those in control group（P〈0.05）, while the expression decreased significantly in PDS ＋ AG group as compared with those in LPDS group（P〈0.05）. Conclusion AG can antagonize the injury of HMr SV5 caused by L-PDS and down-regulate the expression of VEGF.