酸雨和大气污染会对植物叶片内叶绿体的结构和功能产生影响,而植物光诱导延迟发光(DLE)被认为主要产生于叶绿体的PSⅡ中。利用自制的弱光探测系统,以生长周期长、叶片面积大的紫荆花叶片作为实验材料,研究了模拟酸雨和SO2对其DLE特性的影响。结果发现,绿色植物叶片光诱导DLE强度的变化能很好地反映植物叶片中叶绿体完整性以及叶绿体功能的变化。凶此,可以用DLE强度的变化来表征环境对植物的胁迫程度,有望为环境污染监测及生物学评价提供新的手段。
The structure and function of chloroplast in plant leaves can be affected by acid rain and air pollution. The photosystem Ⅱ in a plant is considered the primary site where light-induced delayed-light-emission(DLE) is produced. With lamina of Zijinghua (Bauhinia variegate Linn. ) and soybean (Glycine max(L. )Merr. ) as testing models, we have studied the effects of artificial acid rain and SO2 on characteristics of DLE by using a homemade weak luminescence detection system. The results show that the changes in delayed light emission intensity of green plant can truly reflect the changes in chloroplast intactness and function. With proper calibration, it is likely that DLE may provide an alternative means to evaluate the environmental acid stress on plants. The changes in DLE intensity may provide a new approach for the detection of environmental pollution and its impact on the ecosystem.